Post by Toxics MDPI

📢 #new_publication from Toxics 📝 #Neurotoxic Effects of Aromatic Organophosphate #FlameRetardants Revealed by Lipidomic Analysis in Human Brain Organoids ✍️by Maryam Pyambri et al. Institute of Environmental Assessment and Water Research (IDAEA-CSIC) 🔗https://brnw.ch/21x3MB1 #neural_cells #reactive_oxygen_species #lipidomics 👉This study evaluated phenotypic alterations, such as cell viability, reactive oxygen species generation, and acetylcholinesterase activity, induced by seven widely detected OPFRs in SH-SY5Y human neuroblastoma cells. Aromatic OPFRs such as triphenyl phosphate (TPhP), 2-ethylhexyldiphenyl phosphate (EHDPhP) and tricresyl phosphate (TCP) exhibited the strongest effects, including decreased cell viability, increased oxidative stress and AChE inhibition. Therefore, 3D brain organoid models were used to further explore the potential lipidomic alterations induced by aromatic OPFRs. Lipidomic analysis of brain organoids exposed to aromatic OPFRs (TPhP, EHDPhP and TCP) showed significant alterations across major lipid classes, especially glycerophospholipids, sphingolipids, and glycerolipids. The depletion of bis(monoacylglycerol)phosphate (BMP) species suggests perturbations in endolysosomal lipid homeostasis and membrane trafficking pathways. Increased levels of ether-linked lysophosphatidylcholine (LPC-O) species, together with altered phosphatidylethanolamine (PE) and phosphatidylserine (PS) species, indicate extensive membrane lipid remodeling and changes in cellular signaling.