Post by Corden Pharma - A Full-Service CDMO

Meet our speakers | CordenPharma Peptides Summit 2026 Cell penetration is one of the greatest barriers to developing large molecule therapeutics like peptides, oligonucleotides and proteins. Most large-molecule therapeutics enter the cell through a complex pathway involving endocytosis followed by endosomal escape. This process is so poorly understood and difficult to study that it is challenging simply to measure how much compound is accessing the cytosol. Joshua Kritzer, Professor of Chemistry at Tufts University, will present how the Kritzer Lab has developed new tools to measure cytosolic penetration. The Chloroalkane Penetration Assay (CAPA) is a versatile assay that measures cell penetration using cellularly expressed HaloTag protein and a small chloroalkane tag on the molecule-of-interest. The Kritzer group has used molecular evolution to produce new HaloTag variants which work optimally with a fluorogenic dye. This new “BenzoTag” system allows for turn-on, no-wash cell labeling in seconds. BenzoTag is the basis of a new “turn-on CAPA” which is more sensitive and allows for monitoring cytosolic penetration in real time. 👉 Register now to learn more in Boston: https://lnkd.in/eaV5Cg4N