Post by Christian Feghali

Name: After multiple failed attempts, I’m excited to share this short clip of mitochondria in the Muller G
Uploaded: 2022-09-16T21:31:21.587Z
Channel: Christian Feghali
Description: After multiple failed attempts, I’m excited to share this short clip of mitochondria in the Muller Glia of the zebrafish retina (3dpf). Imaging was done at 1-minute intervals (30 cycles) using airysc

Foundation Year 1 Doctor | University of Cambridge Graduate

After multiple failed attempts, I’m excited to share this short clip of mitochondria in the Muller Glia of the zebrafish retina (3dpf). Imaging was done at 1-minute intervals (30 cycles) using airyscan confocal microscopy with 4Y multiplexing. TP1:mito-mKate construct was injected into Tg(TP1:Venus) embryos to produce retinas where Muller Glia (green) and mitochondria (pink) are fluorescently labelled. Zebrafish were raised under standard conditions at 28°C. Phenylthiourea (PTU) was used to prevent pigmentation.

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