Post by Bio-Works Technologies

📊 A bit of Process Economics for 𝐲𝐨𝐮𝐫 wednesday Todays topic: "𝘏𝘰𝘸 𝘢𝘭𝘬𝘢𝘭𝘪 𝘴𝘵𝘢𝘣𝘪𝘭𝘪𝘵𝘺 𝘪𝘮𝘱𝘢𝘤𝘵𝘴 𝘺𝘰𝘶𝘳 𝘣𝘰𝘵𝘵𝘰𝘮 𝘭𝘪𝘯𝘦" The cost of a chromatography step is 𝐧𝐨𝐭 just about the 𝙞𝙣𝙞𝙩𝙞𝙖𝙡 𝙥𝙧𝙞𝙘𝙚 𝙤𝙛 𝙩𝙝𝙚 𝙧𝙚𝙨𝙞𝙣. It is 𝐡𝐞𝐚𝐯𝐢𝐥𝐲 𝐝𝐢𝐜𝐭𝐚𝐭𝐞𝐝 by process time, buffer consumption, and the frequency of column repacking. 𝐖𝐡𝐞𝐧 optimizing Clean-in-Place (CIP) protocols, evaluating the alkali stability of your resin is a 𝙘𝙧𝙞𝙩𝙞𝙘𝙖𝙡 𝙚𝙘𝙤𝙣𝙤𝙢𝙞𝙘 𝙚𝙭𝙚𝙧𝙘𝙞𝙨𝙚. Here is 𝐰𝐡𝐲:  • 𝐑𝐞𝐝𝐮𝐜𝐞𝐝 𝐝𝐨𝐰𝐧𝐭𝐢𝐦𝐞: A resin that withstands aggressive NaOH cleaning allows for more robust CIP protocols, reducing the risk of carry-over between batches.  • 𝐌𝐚𝐢𝐧𝐭𝐚𝐢𝐧𝐞𝐝 𝐝𝐲𝐧𝐚𝐦𝐢𝐜 𝐜𝐚𝐩𝐚𝐜𝐢𝐭𝐲: High alkaline stability ensures that the dynamic binding capacity (DBC) does not degrade rapidly after a few cycles, allowing you to process the same yields consistently.  • 𝐅𝐚𝐬𝐭𝐞𝐫 𝐩𝐫𝐨𝐜𝐞𝐬𝐬𝐢𝐧𝐠: Resins built for high structural integrity can maintain up to 25% faster flow rates even after repeated cleaning cycles, directly reducing the time spent on the manufacturing floor. Focusing on the long-term structural integrity of your resin is one of the most effective ways to lower the overall cost per gram of purified protein. #ProcessEconomics #DownstreamProcessing #Biomanufacturing #AlkaliStability #CIP