Post by BioDuro

🧪𝗟𝗮𝘁𝗲𝘀𝘁 𝗗𝗠𝗣𝗞 𝗦𝘁𝘂𝗱𝘆: 𝗠𝘂𝗹𝘁𝗶-𝗦𝗽𝗲𝗰𝗶𝗲𝘀 𝗛𝗲𝗽𝗮𝘁𝗼𝗰𝘆𝘁𝗲𝘀 𝗼𝗳 𝗚𝗟𝗣-𝟭 (𝟮𝟴-𝟯𝟲)𝗮𝗺𝗶𝗱𝗲 Our DMPK team recently completed a metabolic profiling study of GLP-1 (28–36)amide to better understand the biotransformation complexity of nonapeptides. Using UPLC-HRMS, we identified 15 metabolites across hepatocytes from five species. While metabolic pathways were largely consistent, we observed clear interspecies differences in metabolite abundance, along with the presence of a rare Phase II glucoside. For oligopeptides around 1,000 Da, rapid proteolytic degradation remains a major hurdle. This study highlights our ability to capture subtle metabolic changes and confirm complex structures across species. By comparing these profiles, we help teams identify metabolic liabilities earlier in development, supporting better-informed decisions and reducing risk before in vivo studies. Download the full study: https://lnkd.in/gdZVNRt7 #DMPK #Peptide #DrugDiscovery #Metabolism #Biotransformation