Post by Asparia Glycomics

Do glycan biomarker studies really need long LC runs and complex labeling workflows? Many glycomics workflows rely on fluorescent labeling followed by LC or CE-LIF, delivering excellent resolution but often requiring extensive sample preparation and long analysis times. But for large biomarker studies — such as IgG glycosylation screening in human serum — what researchers often need is something different: fast, robust and scalable glycan profiling. This is exactly the problem we wanted to address at Asparia Glycomics with the development of CarboQuant™ stable isotope-labelled N-glycan standards, designed as internal standards for MS-based glycan quantification. These standards are now used by many laboratories worldwide and have also been evaluated by major pharmaceutical companies such as Roche and Merck. They support several key applications: • monitoring glycan recovery during sample preparation • quality control of glycomics workflows • method validation and inter-laboratory comparability • quantitative and semi-quantitative glycan biomarker studies Combined with MALDI-TOF MS, this approach enables very fast glycan profiling, making it particularly attractive for studies where large numbers of samples need to be screened. One important example is IgG glycosylation profiling in human serum, which is increasingly explored as a biomarker of: • ageing and longevity • lifestyle changes • chronic inflammation • disease states Using MALDI-TOF MS with isotope-labelled standards, these profiles can be generated rapidly and cost-effectively, without lengthy LC runs or complex fluorescent labeling. At Asparia, we support this workflow end-to-end: • CarboQuant™ isotope-labelled glycan standards • optimized sample preparation from blood samples • MALDI-TOF MS glycan profiling • quantification software This enables one of the fastest and most scalable workflows currently available for IgG glycan biomarker screening. If you are planning large glycomics studies or IgG glycan biomarker projects, we would be happy to discuss how this workflow could accelerate your work. 📩 [email protected] 🌐 https://l1nq.com/UYSNW