Post by Association for Single Cell Analysis (ASCA)
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š RoCK and ROI: single-cell transcriptomics with multiplexed enrichment of selected transcripts and region-specific sequencing Giulia Moro ... Konrad Basler presented RoCK and ROI, a simple and highly versatile scRNA-seq-based method designed to capture specific transcripts and to selectively sequence regions of interest. Ā Ā Key points āļø Targeted capture to enrich for key transcripts āļø The detection and identification of cell types and complex phenotypes āļø Directing a subset of reads to a specific region of interest via selective priming āļø Retrieval of specific sequence information without compromising overall single cell transcriptome information āļø Validation across diverse biological systems ā”ļø More details: https://lnkd.in/dfJYM9sd University of Zurich #SingleCell #SpatialBiology
