Post by abberior

Multiplexing with primary antibodies from the same species has always been a challenge. The 𝗣𝗿𝗲𝗺𝗶𝘅&𝗦𝘁𝗮𝗶𝗻 protocol with Smart Secondary Nanobodies addresses this directly. And here is a 5-color example showing what that looks like in practice. Three targets were labeled using mouse primary antibodies, detected with Smart Secondaries® (𝗙𝗹𝘂𝗼𝗧𝗮𝗴®-𝗫𝟮 𝗮𝗻𝘁𝗶-𝗺𝗼𝘂𝘀𝗲 𝗜𝗴𝗚𝟭) plus direct GFP tag labeling for a fourth channel via 𝗙𝗹𝘂𝗼𝗧𝗮𝗴®-𝗫𝟮 𝗮𝗻𝘁𝗶-𝗚𝗙𝗣. 𝗣𝗵𝗮𝗹𝗹𝗼𝗶𝗱𝗶𝗻 𝗦𝗧𝗔𝗥 𝗕𝗟𝗨𝗘 serves as a structural counterstain for actin. ⬜Actin: abberior STAR BLUE Phalloidin 🟥NUP target 1 (GFP-tagged): FluoTag®-X2 anti-GFP abberior STAR 550 🟩Golgi: FluoTag®-X2 anti-mouse IgG1 abberior STAR ORANGE 🟦NUP target 2: FluoTag®-X2 anti-mouse IgG1 abberior STAR RED 🟧Mitochondria: FluoTag®-X2 anti-mouse IgG1 abberior STAR deepRED These images showcase two new additions to the nanobody conjugate portfolio, developed in collaboration with NanoTag Biotechnologies: 𝗦𝗧𝗔𝗥 𝗱𝗲𝗲𝗽𝗥𝗘𝗗 and 𝗦𝗧𝗔𝗥 𝟱𝟱𝟬 conjugates extend the spectral range of the FluoTag toolbox. #SmartSecondaries #PremixAndStain #STARdyes #FluoTag #abberior #NanoTagBiotechnologies #MulticolorImaging