Washington DC-Baltimore Area
I am passionate about biomedical research and enjoy the intellectual challenge behind it. Since completing graduate school, I have worked exclusively in research departments in various Medical Centers. I want to use my knowledge and skills to assist scientific studies, and in the long run, bring benefits to human lives. I have been working as a lab technician/senior research assistant/lab manager in different research labs, and thus have developed extensive experience in design, develop, perform various experimental procedures and finishing the data analysis. I am able to troubleshoot problems seamlessly and maintain the highest degree of work ethic. My other responsibility is IACUC animal protocols composition/coordination. Besides, I have been assigned a great deal of lab management duties that have given me the opportunity to develop outstanding interpersonal communication skills. I am reliable and enthusiastic to work with people to overcome problems. I am always willing to offer assistance, and is able to follow through to ensure that the work gets done well.
• Played crucial role on carrying out projects on central mechanism and treatment of blast-induced auditory/vestibular injuries. • Successfully accomplished challenging techniques such as the mouse brain ICIV Microinjection of virus vectors, and the trace amount of RNA extraction and real-time qPCR from Rat ear cochlea. • Main duties: Rat/mouse perfusion, fresh tissue collection including Cerebrospinal fluid, blood, brain parts, organs; Brain fresh frozen tissue sectioning using cryostat and microtome; RNA extraction and real-time quantitative PCR; immunohistochemistry-IF on rat/mouse floating and frozen sections; confocal & fluorescent microscope imaging; experimental data summarization and analysis; trouble shooting and optimized various experimental protocols; laboratory material purchasing and organization.
• Main personnel in conducting projects on pharmacological treatments for alcoholic liver disease. • Successfully acting as the coordinator to assist the research department director with administrative duties; also as lab manager for maintaining a well-organized and proficient lab. • Composed and maintained ~10 IACUC animal protocols and executed SRS lab safety regulation. • Main techniques used: Western Blot; ELISA; HPLC (Agilent1200); Immune analysis essay (Fisher Kryptor; Human blood & saliva samples); Paraffin tissue processing and sectioning; IHC, Immunofluorescence; Human cell line culture; Cell-based bioassay (Agilent Seahorse XF Analyzer); transgenic mice breeding, genotyping, and dissection; RNA (mouse liver; human adipose); Real-time qPCR (ABI7900).
• Play the leading role in carrying out projects on effects of subclinical carbon monoxide in developing brain during isoflurane exposure, and anesthesia-induced neurotoxicity in developing retina. • Generated five manuscripts through creating and executing various procedures, and complemented seamless troubleshooting. • Multitasking to keep the lab running efficiently, including personal mentoring/training, purchasing, inventory, organization, and data maintenance etc. • Main techniques used: Mitochondria extraction, Western Blot, Kinetic/heme assays (Agilent Cary300); Blood gas analyzer (Siemens); Enzymatic Assay (human heart biopsy); IHC; IF; Confocal microscope (Zeiss ISM710); transgenic mice breeding, genotyping, and dissection (mouse brain and eyes).
• Effectively worked as a lab manager in a large size (16 members) laboratory and assistant the center director with some research center administrative duties. • Played a crucial role in carrying out research projects on regulation of central nervous system development. • Maintained transgenic mice strains for the whole lab usage. • Coordinated with the writing of about 10 IACUC animal protocols; always follow through to ensure the protocols to be approved as well as renewed yearly. • Main techniques used: DNA/RNA extraction; RT-PCR; Real-time PCR (ABI7900); IHC, IF, Confocal microscope (Olympus); Primary cell culture (Rat embryo progenitor cells); western blots; transgenic mice breeding and genotyping.
• The first member and played crucial role in a starting lab focusing on research projects on traumatic brain injury (TBI) to immature mouse. • Effectively set up and maintained a proficient lab. • Well trained graduate and medical students as well as other lab staff for basic lab techniques. • Developed lab-based programs to keep track of lab supplies, antibodies, and mice breeding colonies. • One of the pioneer researchers working on cDNA microarray gene profiling, and successfully complete hundreds of chips running and analysis. • Collaborated with clinical lab on the drug trial with mouse model. • Main techniques used: DNA/RNA extraction; cDNA Microarray Gene Profiling (Affymatrix, ~400 chips); Real-time qPCR (ABI7700/7900); Laser Capture Microdissection(LCM, Zeiss); Protein extraction; Western Blot; ELISA; IHC, IF; Unbiased Stereology (Olympus CAST); mouse perfusion and brain dissection (>100 mice); mouse brain tissue processing; microtome/cryostat sectioning; transgenic mice breeding and genotyping.
Course: Introductory Biology for Science Major Teach fundamental Lab skills of the science of biology.
Working in the summers as a intern on DNA isolation, PCR, and invertebrate specimen cataloguing.