Singapore
2023 PhD graduate. Investigated the role of the large virulence plasmid in hypervirulent Klebsiella pneumoniae. Techniques learnt include animal models, flow cytometry, PCR (end-point and real-time qPCR), bacterial cloning, Western blot and tissue cell culturing. First author publication in mBio, co-author of 3 other publications. Mentored two undergraduate students for their Final Year Project.
Bacterial Surveillance in Singapore such as Salmonella spp, Carbapenem Resistant Enterobacterales, Vibrio spp, Leptospira spp, Shigella spp, etc using microbiology and molecular testing techniques, including end-point and real-time PCR, serotyping, culturing and whole genome sequencing. Co-section in charge of a team of 7, managing BSL-1 and BSL-2 laboratory operations. Drafted and revised work instructions for proper documentation of laboratory test protocols, to conform to ISO 15189. Verified test results and meeting turnaround time for reporting of statistics to MOH. Coordinated and supported bacterial foodborne outbreak investigations with MOH.
- Investigated the role of large virulence plasmid in hypervirulent Klebsiella pneumoniae fitness and pathogenesis - Discovered a novel iron-dependent regulation of the Type 3 Fimbriae through an uncharacterised small protein, affecting biofilm, cell adhesion and in vivo gut colonization. - Mentored two undergraduate honours students for their Final Year Project, both attained high grades and continued Ph.D. studies, with my first student awarded the President's Graduate Scholarship and my second student awarded the Sim Kit Peng Honors Book Price for outstanding project achievement. - Conferences attended: Cold Spring Harbor Laboratory Microbial Pathogenesis and Host Response (New York, Sep 2019) and International Symposium on Plasmid Biology Meeting (Toulouse, Sep 2022). - 1 first author and 2 co-authored publications
Taught English and Science from Primary 2 to Primary 4 level at Punggol View Primary School. Co-coached students in floorball together with teachers in charge and instructor to promote the sport and develop students’ interests.
Facilitated overseas and local delegates' hospitality arrangements during the Singapore Air Show 2014.