Orindiúva, São Paulo, Brazil
- Biotechnologist with 8+ years of experience in the genetics of plant-pathogen interaction and crop genetics improvement; - It includes elaboration of research projects, planning and conducting experiments, training and supervising junior staff; - Strong background in molecular genetics and plant biotechnology techniques including: molecular cloning, gene expression analysis, confocal and fluorescence microscopy, plant transformation and phenotype analysis - Experience applying Good Laboratory Practices (GLP) and working in a certified laboratory (ISO9001)
Plant genetic transformation aiming resistance to drought and heat
Advisor: Dr. Alessandra Alves de Souza Project: Functional and applied study of RAP2.2 transcriptional factor and RPS5 resistance gene in Xylella fastidiosa resistance (FAPESP/CAPES Fellowship) Main activities included: - 3D protein structure analysis (in silico) - C. sinensis transformation - Severity analysis of transgenic plants (Arabidopsis and citrus) infected with X. fastidiosa - Gene expression analysis (qPCR) - Pathogen detection (PCR) and quantification (qPCR)
Advisor: Dr. Alessandra Alves de Souza Project: Nicotiana tabacum and Arabidopsis thaliana as models plants for functional study of genes associated with resistance to citrus variegated chlorosis (FAPESP Fellowship) Main activities included: - Tobacco inoculation with X. fastidiosa strains and symptom assessment by software (ImageJ) - Diagrammatic scale for symptom's severity - Selection and screening of A. thaliana mutants infected by X. fastidiosa - Fluorescence microscopy of A. thaliana mutants infected by X. fastidiosa-GFP - Pathogen detection (PCR) and quantification (qPCR)
Supervisor: Dr. Maeli Melotto (CNPq Fellowship) Main activities included: - Cloning plasmid vectors - Arabidopsis transformation - Improving the Arabidopsis-Xylella interaction knowledge to enhance functional studies - Yeast Two-Hybrid library screening (Protein-protein interactions) - Transient expression in Nicotiana benthamiana (Subcelullar localization) - Confocal and fluorescent microscopy
Advisor: Dr. Alessandra Alves de Souza (FAPESP Fellowship) Main activities included: - Citrus transformation - Tobacco transformation - Pathogen detection (PCR) and quantification (qPCR)