Rivas-Vaciamadrid, Community of Madrid, Spain
Laboratory Technician
Mouse colony management (crosses, weaning and marking) Experiments with CAR-T cells, injecting tumor cells intravenously and monitoring by ivis fluorescence techniques Management of the patient biobank Cell culture
Behavioral behaviors (odor and object recognition, haging wire, rotarod) Necropsy extracting all tissues for analysis by western blot and qPCR Experience in histology, sample freezing, cryostat cutting and immunohistochemistry. DNA & RNA extraction Cell culture
Mouse colony management (crosses, weaning and marking) and genotyping of muscular dystrophy mouse models. Experience in histology, sample freezing, cryostat cutting and immunohistochemistry. Mouse necropsies removing muscle tissue for digestion and subsequent cytometry processing. Administration of drugs by oral probing, realization of the treadmill and bleeding for ELISA.
Behavioral behaviors (NOL, PPI, Actimetro and Nest Building). Experience in histology, sample freezing, cryostat cutting and immunohistochemistry. Stereotactic surgeries implanting brain cannulas. Mouse necropsies perfusing and extracting the brain.
Mouse colony management (crosses, weaning and marking) and genotyping of diabetic and high-fat diet mouse models. Mouse necropsies, surgery procedures and mice fetus isolation. Histology: experienced in microtome slicing of paraffin blocks and immunohistochemistry. Cannulation of digestive solutions for the extraction of liver and pancreatic tissue and posterior in vitro culture of hepatocytes and pancreatic islets.