Mountain View, California, United States
Develop biochemical assays and experimental workflows supporting the development of next-generation sequencing technologies. Collaborate across research teams to generate high-quality data, improve assay performance, and support product development. •Developed a high-throughput 96-plex protein purification and formulation workflow that significantly increased screening capacity. • Led a cross-functional subgroup supporting off-sequencer assay development. • Routinely design and execute biochemical fluorescence-based and label-free assays to evaluate enzyme activity, kinetics, binding affinity, and molecular interactions. • Analyze and communicate experimental results to inform technical decisions and project direction. • Mentor scientists, establish experimental best practices, and contribute to a collaborative research environment. Core expertise: Assay Development • Protein Purification • Enzyme Kinetics • Fluorescence Assays • High-Throughput Screening • Laboratory Automation • Data Analysis • Cross-Functional Collaboration
-Actively played a role in establishing the Stony Brook Consulting Club. -Consulted pro-bono for a life sciences company preparing to enter a unique market.
The chemical biology training program (CBTP) provided a platform to both provide and receive feedback from colleagues in a collaborative setting where we are trained to effectively communicate our ideas. I frequently led presentations as well as offered structural biology-related input towards various projects.
Project Title: Understanding the effects of histone modification on chromatin structure and function. Technical skill set: -Protein purification -FPLC (AKTA) with Unicorn -Structural determination/characterization -Negative-stain electron microscopy -Cryo-EM -Insect cell culture -EMSA -Biochemical assays Software: -Relion -cryoSPARC -EMAN2 -PyMol -COOT -Chimera -ImageJ -GraphPad
Thesis Titled: Developing a system to determine the ubiquitin-substrate spectrum of an E2 enzyme Technical skill set: -Molecular cloning -Yeast culture -Western blotting -X-ray crystallography
Technical skill set: -PCR -Site-directed mutagenesis -Plasmid preparation and purification -SDS-PAGE -Agarose gel electrophoresis.
-Led lectures for two organic chemistry lab sections -Maintained and updated the chemical stock room -Provided oversight and managed teaching assistants