Tsotne Chitiashvili

Scientist at Calico Life Sciences

South San Francisco, California, United States

About

Experience

  • Calico Life Sciences (4 yrs 6 mos)
    • Scientist II
      Aug 2023 - Present · 3 yrs

    • Scientist
      Feb 2022 - Aug 2023 · 1 yr 7 mos

  • University of California, Los Angeles (Los Angeles, California, United States)
    • PhD
      Sep 2016 - Feb 2022 · 5 yrs 6 mos

      In the laboratories of Dr. Amander Clark and Dr. Kathrin Plath we were working on X chromosome dynamics during human early embryonic development. Using single cell technologies together with microscopy we have dissected molecular mechanisms of sex specific differences taking place during human germline formation.

    • Teaching Assistant
      Sep 2020 - Dec 2020 · 4 mos

      Teaching assistant of upper division class - Developmental Biology

    • Teaching Assistant
      Jan 2018 - Apr 2018 · 4 mos

      Teaching assistant for upper division class - Molecular Biology of Cellular Processes.

  • Ludwig-Maximilians-Universität München (2 yrs 1 mo)
    • Graduate Researcher
      Jan 2015 - Oct 2015 · 10 mos

      As a Master student in Prof. Heinrich Leonhardt's lab we have employed 3D-SIM Super Resolution imaging to investigate the role of repeat elements of lncRNA Xist in silencing X-linked genes. Using this approach we have visualized, quantified and analyzed Xist molecules in wild type and and mutant cell lines lacking repeat elements in the Xist transcript. Ultimately this work has contributed to the Nature paper: "A protein assembly mediates Xist localization and gene silencing".

    • Teaching Assistant
      Jul 2015 - Aug 2015 · 2 mos

      Teaching assistant for Bio-imaging practical course for 2nd year Master students.

    • Graduate Researcher
      Oct 2014 - Feb 2015 · 5 mos

      In Leonhardt lab, I have worked to develop CRISPR based targeted gene silencing tools

  • Undergraduate Researcher at Max Planck Institute for Biochemistry
    Feb 2013 - Jun 2013 · 5 mos

    I worked on my Bachelor Thesis with Prof. Frank Schnorrer's lab investigating the role of Dlg5 in Drosophila flight muscle development. Using RNAi crosses we have shown that Dlg5 plays essential role in flight muscle development and ultimately its knockdown results in lethality during pupal development.