San Mateo, California, United States
Perform skilled mechanical and analytical duties on development of new vaccine for resistant bacteria causing Tuberculosis that include but not limited to culturing eukaryotic cells, cloning experiments, culturing bacteria, transformation, procedure, and animal work. In addition, conducted a research on effect of Methionine Sulfoxide Reducase (Msr) A enzyme on cytotoxicity. • Cultured and infected HeLa, CRL, and raw cell lines with Mycoplasma genitalium. • Analyzed for cytotoxicity under confocal laser scanning microscopy by capturing differential interference contrast (DIC) • Purification of recombinant Mycoplasma pneumoniae MsrA from E-coli • Conducted Reactive Oxygen Species test using Fluorescent probe 2’, 7’- Dichlorodihydrofluorescein Diacetate (DCF-DA) • Detection of cell signaling using Western Blot analysis • Determined Necrotic and apoptotic cells during infection and treatment of MsrA using Promokine kit • Analyzed TNF-alpha signaling level by ELISA test using Human TNF-alpha ELISA Ready-Set-Go Kit (eBioscience)
Performs variety of experiments that support research and development activities in various disciplines by operating scientific equipment, selecting appropriate methods and techniques to perform experiments, analyzing data and preparing related reports. • Completed Training for High Performance Liquid Chromatography (HPLC), Ultra Performance Liquid Chromatography (UPLC), Karl Fischer (Volumetric and Oven-attached) titration, Spectrophotometer, Supercritical Fluid Chromatography (SFC), Dissolution instruments, Infrared Spectroscopy (IR), and Ion Chromatography, Gas Chromatography (GC), and ICP • Provide support for Process chemistry, pilot plant operation through sample analysis, method validation, and data review. • Support API and drug product development studies for the assigned project and develop analytical methods as necessary • Trained on Empower software to analyze data. • Attend Seminars and instrumental training of operational concepts and instrument troubleshooting • Support Management of Laboratory instruments and equipment, and partake in the Safety team that insures Laboratories are GMP complaint.
Fully trained in both qualitative and quantitative Microbiology departments to prepare, enrich, pipette, and plate various food samples to be tested and analyzed for pathogens. • In charge of setting up and testing quality control samples each week. • Revised and improved the Salmonella pH system. • Operating VIDAS® Salmonella and Listeria for detection Salmonella and Listeria pathogens in food samples
Test the effect of cigarette smoke exposure on plasma insulin, cortisol, and macrophage effect on mice by exposing them to first-hand cigarette smoke and obtain blood sample from each mice. • Analyze blood insulin level by quantifying it using Insulin-ELISA protocol • Determine Cortisol of mice fluids using the Assay Designs' Correlate-EIA tm Cortisol kit