Oxford, England, United Kingdom
Postdoctoral research assistant specialized in the biology of ageing and longevity, working at the department of Biochemistry of the university of Oxford, in the team of Prof. Lynne Cox, on cell senescence in bones in the context of type 1 diabetes. PhD work involved the biology of ageing, inflammation, glycation and the Receptor for Advanced Glycation End-products (RAGE).
Postdoctoral research assistant in bone cell senescence in type 1 diabetes. Principal Investigator: Professor Lynne S. Cox. In collaboration with Professor Rivka Dresner Pollak team, Hadassah Medical Center, Israel.
PhD in the Lille Inflammation Research International Center (LIRIC) in Lille. Currently working on Advanced Glycation End-products (AGEs) and their main receptor, the Receptor for AGEs (RAGE) and their impact on (renal) ageing in mice. Skills: Immunohistochemistry, Immunofluorescence, Enzyme-linked immunosorbent assay (ELISA), Dot Blot, Western Blot, RT-qPCR, LC-MS/MS, Flow Cytometry, Cell Culture, Mesangial Cell Isolation, Mouse Handling, Metabolic Cage, Physical Activity Assays. Principal Investigator: Professor Eric Boulanger.
Lifespan and healthspan study of Caenorhabditis elegans in various genetic backgrounds involving wrn-1 (Werner Syndrom), cep-1 (p53 ortholog), daf-16 and age-1 (daf-2 (insulin) pathway). Technics: Global C. elegans handling, lipofuscin analysis, RNAi, PCR, RT-PCR, vector modification, bacterial transformation, minipreps, DNA purification, Rapid Translation System (RTS), protein purification, Co-Immunoprecipitation, Western blot, Southern blot. Principal Investigator: Professor Lynne S. Cox. Étude de la longévité et de la santé associée du vers Caenorhabdtis elegans wild type et dans divers contextes génétiques en lien avec Wrn-1 (protéine mutée menant à un Syndrome de Werner), cep-1 (orthologue de p53), age-1 et daf-16 (voie de daf-2 (insuline)). Techniques : Manipulations générales de C. elegans, analyse de lipofuscine, ARNi, PCR, RT-PCR, modification de vecteurs, transformation de bactéries, minipreps, purification d'ADN, Rapid Translation System (RTS), purification protéique, Co-Immunoprécipitation, Western blot, Southern blot. Maître de stage : Professeur Lynne S. Cox.
Identification of surface markers of muscle satellite cells, myoblasts and myotubes of mice, dogs, non human primates and humans. Study of aldehyde dehydrogenase positive cells which have a very good myogenic profile. Skills: Cell culture (lines and primary cells), flow cytometry and immunocytofluorescence. Principal Investigator: Jean Thomas Vilquin. Caractérisation de marqueurs de surface de cellules satellites, de myoblastes et de myotubes de souris, de chien, de primates non humains et d'humain. Étude parallèle des cellules Aldéhyde Déshydrogénase (ALDH) positives. Perspective de thérapie cellulaire du muscle squelettique. Maître de stage : Jean Thomas Vilquin.
Endoplasmic Reticulum stress analysis through the induction of the Unfolded Protein Response in multiple myeloma cell lines. Skills: Non-adherent cell culture, MTT assay, Flow cytometry. Supervisor: Patricia Gomez; Principal Investigator: Martine Amiot. Étude de l'induction du stress du Réticulum Endoplasmique via la voie UPR (Unfolded Protein Response) sur des lignées du Myélome Multiple. Maître de stage : Martine Amiot; Responsable : Patricia Gomez.