Lausanne, Vaud, Switzerland
Experienced Research Technician with a demonstrated history of working in Life Science. Skilled in Cancer Research, Cell Culture, Gene Expression, Mice Trial, Stem Cells, Flow Cytometry and Histology. Strong research professional graduated from Ecole de Laborantines médicales, Genève.
In the group of Professor Douglas Hanahan. Currently working with Dr. Krisztian Homicsko on the melanoma’s project. Mice trial ( Tumor induction, oral gavage, Intra Peritoneal injection, Subcutaneous Injections, tail vein injection, dissection, hemogramm) Histology ( embedding, microtome cutting, staining and immunohistochemistry, slide scanner) Gene expression and NGS ( RNA extraction, RT and qPCR, single cells using 10x Genomics. Library production. Protein (Extraction, BCA and Bradford, Western-blot, ELISA) Cell culture ( Stable lines and primary cell lines from human and mouse melanoma, isolation of macrophage from bone marrow, transfection).
Research assistant in the group of the Professor Michel Aguet. Responsible of the mice colony, maintenance of the laboratory. Ordering consumable. Participate of the project BCL9/9L in colon cancer. Mostly working with lentivirus, flow cytometry to isolate stem cells population, tissue culture, RNA extraction, Reverse transcription and qPCR. Microdissection. DNA extraction and genotyping. Protein extraction. Jürgen Deka; Norbert Wiedemann; Pascale Anderle; Fabienne Murphy-Seiler; Jennyfer Bultinck; Sven Eyckerman; Jean-Christophe Stehle; Sylvie André; Nathalie Vilain; Olav Zilian; Sylvie Robine; Mauro Delorenzi; Konrad Basler; Michel Aguet. Bcl9/Bcl9l are critical for Wnt-mediated regulation of stem cell traits in colon epithelium and adenocarcinomas. Cancer research 2010;70(16):6619-28.
Worked for the Professor Richard Iggo. Involved mainly in the study of the EORTC 10994/BIG 00-01 clinical trial. Cryostat, H&E staining, RNA extraction, Reverse Transcription, PCR, sequencing, Yeast transfection. Microarray (Affymetrix) Hervé Bonnefoi; Etienne Brain; David A Cameron; Tanja Cufer; Pierre Fumoleau; Richard D Iggo; Jacek Jassem; Lissandra Dal Lago; Elisabet Lidbrink; Saskia Litière; Alain Lortholary; Louis Mauriac; Thierry Petit; Martine Piccart; Philippe Rouanet; Khalil Zaman; Sylvie André; Véronique Becette; Jonas Bergh; Emmanuel Blot; Jan Bogaerts. TP53 status for prediction of sensitivity to taxane versus non-taxane neoadjuvant chemotherapy in breast cancer (EORTC 10994/BIG 1-00): a randomised phase 3 trial. Lancet Oncol. 2011 Jun;12(6):527-39. doi: 10.1016/S1470-2045(11)70094-8
Moved the lab with the Professor Richard Iggo in St Andrews in Scotland. Stephan Duss, Sylvie André, Anne-Laure Nicoulaz, Maryse Fiche, Hervé Bonnefoi, Cathrin Brisken and Richard D Iggo. An oestrogen-dependent model of breast cancer created by transformation of normal human mammary epithelial cells. Breast Cancer Research 2007, 9:R38
Worked in the group of Professor Richard Iggo. Mainly involved in a study of the EORTC 10994/BIG 00-01 clinical trial. Also experienced in cell culture and adenovirus production. Farmer, Pierre; Bonnefoi, H; Anderle, Pascale; Cameron, David A; Wirapati, Pratyaksha; Wirapati, Pratyaksha; Becette, Véronique; André, Sylvie; Piccart-Gebhart, Martine; Campone, M.; Brain, Etienne; MacGrogan, G; Petit, Thierry; Jassem, J.; Bibeau, Frédéric; Blot, Emmanuel; Bogaerts, J; Aguet, Michel; Bergh, Jonas; Iggo, Richard; Delorenzi, Mauro. A stroma-related gene signature predicts resistance to neoadjuvant chemotherapy in breast cancer. Nature medicine, 15, 1, (page 68-74)