Greater Boston
* Interdisciplinary knowledge in the life science sector, with expertise on genomics, genetics, molecular, clinical, biochemistry, microbiology, cellular, plant, and synthetic biology. * Over 15 years of experience in genetic, genome engineering and advanced molecular biological techniques, including vector designs/delivery, PCR/qRT-PCR/ddPCR, plasmid/gDNA/cfDNA/RNA purification, IVT/protein expression/purification, cell-based assays, flow cytometry, HPLC-LC/MS, and sterile culture techniques. * Proficient in Illumina (Miseq/Nextseq/Novaseq6000DX)/Ion Torrent platforms, NGS/bioinformatic for RNAseq, ctDNA, and amplicon-based sequencing, and automation platform using AutoDG/Kingfisher/Maxwell/Genexus/Hamilton. ------------------------------------------------------------------------------------------------------ My doctoral research was focusing on genetics and specific metabolism in plant, especially with particular interest in lignin biosynthesis of plant cell wall. I had characterized monolignol biosynthetic enzymes by protein purification and enzyme/inhibition kinetics using HPLC and MS. I had performed protein-protein interaction using immunoprecipitation and BiFC on confocal microscopy. Also, I was able to perform plant tissue culture for plant transformation. My master research was focusing on HBV-induced liver cancer. Using liver-specific promoter and inducible regulatory cassette, a conditional expression system in the liver of zebrafish was established. The inducible GFP expression was observed under florescent microscope and the oncogenesis of HBx, a putative oncogene in the genome of HBV, was studied. In NREL, I focused on tailoring bioenergy crops, switchgrass, to improve the thermo-chemical conversion efficiency of herbaceous biomass for biofuel production. In addition, I studied plant-microbe interactions, especially plant growth promoting bacteria, for improving biomass yield. In LBNL, I am focusing on tailoring bioenergy crops by synthetic biology and metabolic engineering to improve the yield of biomass and bioproduct production. During my career, I had been taught and inspired by great researchers and my career goal is to also become a scientist that can devote on the biological problems in our life and shred lights on those unsolved questions.
Leading a team of scientists to understand novel engineering pipeline using synthetic biology and strengthen company's IP portfolio.
Applied synthetic biology to enable metabolic engineering towards circular bioeconomy.
Studying the effects of bio-catalysts in plant biomass for improving the biofuel production.
Studying the molecular regulation of lignin biosynthesis.