Ipswich, Massachusetts, United States
RNA biologist and Protein biochemist at New England Biolabs, Inc. • Successful research scientist with strong publication record in high impact peer-reviewed journals • Focused on RNA biology, including in vitro transcription, RNA modifications (targeted modifications and detection) and Ribonucleases • Extensive experience in protein engineering, purification, and characterization • Broad experience in biochemical, molecular biology, and genomic technologies • Successful independent project planning • Excellent communication and writing skills in English and German
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• Co-established ChEC-seq, a novel genomic method using in situ chromatin endogenous cleavage (ChEC) by fusion proteins, followed by high-throughput sequencing (NGS) for background-free genome-wide identification of binding sites of chromatin associated factors. (Grünberg, S., Zentner, G. E. Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae. J. Vis. Exp. (124), e55836, doi:10.3791/55836 (2017)) • Used protein crosslinking coupled with mass spectroscopy (CX-MS), involving high-performance liquid chromatography (HPLC), to identify protein-protein interaction interfaces. • Developed a novel technology coupling in vivo site-specific protein-protein crosslinking with mass spectroscopy (MS) to identify protein interactions in RNA polymerase II transcription initiation complexes.
• Analyzed protein-protein interactions using multiple technologies (Fast protein liquid chromatography (FPLC), Protein-Protein Crosslinking, mutational studies, Immunoprecipitation (IP)). This work lead to the discovery on how DNA opens at the very beginning when a gene is switched on. • Modified and purified megadalton sized, multi subunit protein complexes with subsequent identification and characterization by mass spectroscopy (MS). • Designed and purified hybrid fusion proteins of a general transcription factor for crystallization. • Modeled unknown protein structures using state of the art software in combination with biochemical data. • Collaborated with, among others, Dr. Patrick Cramer (Max Planck Institute, Göttingen, Germany), Dr. Jeff Ranish (Institute for Systems Biology, Seattle, WA), Dr. Eva Nogales (UC Berkley, CA) • Trained and supervised national and international scientists with diverse skills and backgrounds, among others as part of an international student exchange program by the DAAD (German Academic Foreign Affairs).
• Engineered hyperthermic archaeal proteins and protein complexes to allow for handling under aerobic and medium temperature conditions without loss of function. • Designed and set-up a novel protein purification pipeline, for the first time enabling the reconstitution and purification of a hyperthermophilic multi-protein complex with subsequent function analysis. • Completed GE Healthcare's training on troubleshooting ÄKTA design systems, UNICORN control software, and the purification of recombinant proteins.
• Completed practical training in immunology (among others: analysis of blood serums, work with human cell lines) and various electrophoresis techniques.