Boston, Massachusetts, United States
Advisor: Andrew Kruse, Ph.D. Used protein engineering to improve the biochemical and pharmacokinetic properties of the peptide hormone relaxin-2, resulting in patent filing and out-licensing. Determined the active-state structure of the G protein-coupled receptor RXFP1 using cryo-electron microscopy. Studied RXFP1's signaling mechanisms using receptor mutagenesis, flow cytometry, and cell-based signaling assays.
Tested bispecific antibodies through cell-based assays, including ELISAs, flow cytometry, and cell viability assays.
Examined the regulation of the Wrap53 gene in response to DNA damage through transcriptional reporter assays and quantitative reverse transcription PCR.
Studied the enzyme acyl protein thioesterase I through protein purifications and siRNA knockdown and overexpression studies.