Mannheim, Baden-Württemberg, Germany
Signal Transduction in Cancer and Metabolism / Dr. Aurelio Teleman • Cultivation of mammalian cells (MCF7, HeLa, U2OS, Hek 293T and Jurkat) • Cell transfection for overexpression or knock down of GOI • Dual Luciferase reporter gene assays in mammalian cells to study the effect of gene knockdown, depletion of nutrients or treatment with pharmacological active compounds • CRISPR/Cas9-mediated gene editing in mammalian cells • Generation of stable mammalian cell lines expressing GOI • Expression and purification of recombinant proteins in E.coli • Broad range of molecular biology techniques including: Cloning, different PCR techniques (e.g. site-directed mutagenensis, Gybson Assembly), DNA and RNA extraction from tissue and cultured cells, cDNA synthesis, qPCR, DNA Sequence analysis, Gel ectrophoresis of DNA and RNA, Western Blot, IP • P-element, PhiC31 and CRISP/Cas9 mediated germ line transformations of D. melanogaster • Lab managemant duties
Developmental Biology / Dr. Anne Ephrussi Main focus: Drosophila Melanogaster/ Cell polarity and RNA localisation • Responsible for the EMBL Drosophila Transgenic Service - Microinjection in Drosphila Embryos - P-element, PhiC31 and CRISPR/Cas9 mediated transgenesis. • Maintenance of D. melanogaster stock collection • Molecular Biology (PCR, Cloning, Western Blot) • Flourescence In Situ Hybridisation (FISH) and Immunostainings of D. melanogaster Ovaries • Epi-flourescence and Confocal microscopy • Y2H Assays • Protein expression and purification in E.Coli for Crystallization and Structure Analysis • Lab organisation
Developmental Biology / Dr. Stephen Cohen Main focus: Drosophila Melanogaster / Growth and Metabolism • Responsible for the EMBL Drosophila Transgenic Service - Microinjection in Drosophila Embryos - P-element mediated transgenesis • Molecular Biology • Lab organisation
Assay Development and Reagent Production / Babette Aicher, Ph.D. • Development, performance and analysis of biochemical and cell based kinase assays for identification and optimization of pharmacological active compounds • Expression of recombinant proteins in E.coli • Purification of proteins and antibodies
Target Identification / Dr. Günter Brönner • Setup of phenotypic screens based on classic Drosophila genetic • Development of biochemical high-throughput triglyceride analysis for phenotypic characterisation of Drosophila lines
Department of Plant Pathology and Crop Protection Generell Plant Pathology and Plant Protection / Dr. Birger Koopmann • Resistance testing of brassica napus against pathogenic fungi • Analysis of phytoalexins in plant and herbicide residues in soil by HPLC Agricultural Entomologie / Dr. Bernd Ulber • Breeding and care of plants for experiments • Design, processing and analysis of laboratory, greenhouse, and outdoor experiments • Maintenance of insect stocks