Brussels, Brussels Region, Belgium
laboratory technician with experience in biotechnology, academic research, and the pharmaceutical industry. Skilled in molecular biology, microbiology, cell culture, and protein purification, I am proficient in a wide range of experimental and instrumental techniques. I also have solid experience in laboratory operations management, including stock and supply oversight, equipment maintenance coordination, and supplier interaction. Rigorous, autonomous, and familiar with GMP environments, I ensure the quality, reliability, and traceability of all analyses. 🧪 Technical Skills • Cell culture: human, bacterial, and yeast cell lines; sterile work without antibiotics • Molecular biology: PCR, RT-PCR, cloning, DNA/RNA/protein extraction and purification • Microbiology: bacterial and yeast transformations, MIC testing, biofilm studies • Protein purification: LPLC systems (AKTA Start) • Protein analysis: SDS-PAGE, Western blot, Bradford • Instrumentation: HPLC, spectrophotometer, autoclaves, incubators • Laboratory management: ordering, inventory, maintenance coordination, supplier management • Languages: French (native), English (professional)
I worked at UCLouvain Drug Research Institute. My role involves supervising students during internships and guiding doctoral candidates through laboratory experiments. My skills include handling cell cultures, preparing plates, performing intracellular transformations, conducting Minimum Inhibitory Concentration (MIC) tests, managing bacterial cultures, studying biofilms, and separating internal and external membranes of bacteria. Furthermore, I am engaged in molecular biology tasks such as DNA, RNA, and protein extraction, purification, and quantification, as well as advanced techniques like Western blot, PCR, RT-PCR, HPTLC, and HPCL. I am also responsible for operating and maintaining advanced equipment such as HPLCs, autoclaves, and incubators. Besides my lab duties, I oversee material stock management, analyse, and present research results. Skills: Molecular Biology · Microsoft Word · HPLC · Biochemistry · PCR · English · Microbiology · Analytical Skills · Animal Handling · LabTech · DNA Extraction · Teamwork · Microsoft Excel · Western Blot · ELISA · Basic Research · Molecular Microbiology · In Vivo · RNA Extraction · Laboratory Techniques · Analytical Chemistry · Laboratory Skills · Microsoft PowerPoint · Good Laboratory Practices · Microscopy · RT-PCR · Laboratory Technicians · In Vitro · Logistics Management · Cell and Molecular Biology · Laboratory Animal Medicine · Cell Culture · Gas Chromatography and Mass Spectrometry · Versatility · Cell Biology · Microsoft Office · Protein Extraction · Plasmid DNA Purification · Validation · Office Automation · Fluorescence Microscopy · Animal Handling
I was responsible for running tests according to the schedule provided by my supervisor. After incubation, I counted the boxes and entered all results into the dedicated computer systems. I worked closely with my team and checked consumables regularly to avoid any stock shortages. I also took care of equipment maintenance, making sure everything worked correctly and followed good laboratory practices. A big part of my job involved membrane filtration tests and microbial strain identification, which were essential for checking the quality and safety of the products. I made sure these tests were done correctly and on time, following all the required procedures. When I obtained out-of-specification results, I started the investigation and followed the process until the end. I always respected analysis and release deadlines and helped remind my colleagues about the importance of GMP rules. I also contributed to maintaining the laboratory equipment and improving the workspace. I reported any safety or hygiene issues and used Quality of Production (QLP) and Environment, Health, and Safety (EHS) tools to help improve our daily work. Finally, I kept my certifications up to date to ensure the quality and compliance of everything I did.In case of out-of-specification results, I initiated investigations and followed the process through. I met the deadlines for analysis and release, while raising awareness among my colleagues about Good Manufacturing Practices (GMP). I participated in maintaining laboratory equipment and facilities, with a focus on enhancing our work environment. I communicated any anomalies related to safety and hygiene and actively contributed to improving our workplace by utilizing Quality of Production (QLP) and Environment, Health, and Safety (EHS) tools. Furthermore, I maintained my own certification to ensure the quality and compliance of my work.
I provided lab support in the Molecular Biology/Microbiology Research section at UCB Pharma S.A. My duties involved managing cell lines and conducting molecular biology experiments related to DNA, RNA, and proteins.
During my internship, I focused on studying amino acid excretion in Saccharomyces cerevisiae. Specifically, I investigated how the deletion or retention of transport proteins influenced the yeast's assimilation of amino acids. Additionally, I constructed a specialized plasmid for genetic transformation experiments in yeast cells. This hands-on experience honed my laboratory skills and deepened my understanding of genetic processes and cellular behavior.