Colombier, Neuchâtel, Switzerland
Specialities : Development of cell-culture processes at small scale (WP, SF, Ambr 15) and small-scale bioreactors, cellular Biology and metabolism, fedbatches. Use of statistical sofware (DOE, Modelisation)
- USP tech transfer lead - Tech transfer lead (project manager)
- USP tech transfer lead and manufacturing support - PPQ run and file writing - Tech transfer lead (project manager)
-Tech transfer support: USP lead for implementation of an upstream process in the facility (Scale-down and at scale) - MSAT lab: starting of the lab and implementation of scale down model - Manufacturing support
- Medium development activities (CHO cells) - Small-scale cell culture process development (shake-flask, Ambr15 to benchtop bioreactor)
- In charge of medium development activities (CHO cells) - Small-scale cell culture process development (shake-flask, Ambr15 to benchtop bioreactor)
-> Production (wave bags) and confirmation runs (SUB50L and 250L); Clarification using STAX or POD. -> CHO cell culture at small scale (SF125, Ambr 15, Lab scale bioreactor) -> Implementation of Ambr15 in the upstream process development platform.
Development of a fermentation process of plasmids at 10L scale : The production of plasmids is a limiting step in the processes using transient transfection in mammalian cells for recombinant production and viral vectors but also to produce DNA vaccines and plamids for gene therapy. The eventual aim of this project is to develop a process to produce R & D batches of purified plasmids at a 10l scale. The availability of this method internally would remove this limitation, at least for development stages. Activities : - Search and study of the litterature in the field of plasmid production with E.Coli. - Establishment of Batch and fed-batch process to develop high yield production process with Sartorius Biostat - BPlus bioreactor at 2L scale/Optimisation of processes - Establishment of analytics tools (Molecular Biology) In parallel, cell culture studies at small scale (T-Flasks, Shake-Flasks ans bioreactor). Personal qualities : rigour, autonomy
R&D studies with the low field NMR technique on food and cometic products : Low-field NMR characterizes the compartmentalization of protons and the texture of samples. The obtained data after direct analysis or confrontation with other biological parameters will identify quality markers and improve the manufacturing process or the product formulation. During this internship, I was in charge of several projects of low-field NMR on products in food and cosmetic industries. The first selected study for this report deal with the preservation of an emulsion and the second determine the influence of raw material nature on chocolate syrups texture. Activities : -Behavior analysis of products depending on serveral parameters as temperature preservation, pH, composition. -Conducting studies, results analisys, report writing. -Water analyses