Dobbs Ferry, New York, United States
• Biochemical Scientist with a Master’s in Biomedical Engineering and 2+ years of industry experience in immunoassay development and verification. • In-depth practical knowledge in the field of high-throughput chemiluminescent immunoassays and immunological techniques like ELISA, sandwich, and competitive assays, etc. • Excellent independent and team player, collaborative with capability of multitasking and effectively prioritizing critical, time-sensitive assignments; fast learner with high-adaptability and demonstrated capabilities of excelling in high-pressure environments. • Proficient in handling lab equipment and instruments, pipetting, performing dilutions, handling infectious samples etc. • Extensively strategic, detail oriented, creative, and quality oriented approach to work.
Assay Verification Experience: • Responsible for planning and executing verification studies on Early Engineering Prototype (EP) of newly designed Atellica CI Multifunctional High-throughput Immunoassay analyzer to evaluate and verify product claims of assays, relative to Design Input Requirements (DIR) goals or pre-established assay performance claims for marketing purpose. • Assays worked on: Prostate Specific Antigen (free, complexed, and total), Digitoxin, Hepatitis B (Surface II Antigen and Confirmatory) • Studies independently conducted: Precision, In-Use Life Stability: Calibration Interval and Reagent Onboard Stability, Analytical Sensitivity, Functional Sensitivity, Limit of Blank/Detection/Quantitation, Assay Linearity, Assay Hook Effect, Manual vs. Automated dilution. Studies assisted with: Control Systems Evaluation, Method Comparison. • Proficient in operating: Atellica CI and IM, ADVIA Centaur XP and CP immunoassay analyzers. • Constructed serum and plasma assay panels for LOBD/Q, Precision, Functional sensitivity studies. • Responsible for weekly and monthly maintenance and safety of assigned instruments. • Conducted and updated inventory of assay reagents, calibrators, controls, samples, and other project supplies monthly. • Crunched study data using in-house data crunching tool, analyzed, tabulated in Excel, and presented data in daily group meetings. • Prepared study specific Electronic Lab Notebooks for report writing and FDA submissions. • Documented study results of assigned studies in Azure DevOps for maintaining audit trail and tracking project progress. • Resolved and documented common system issues and escalating major mechanical failures to service engineers. • Requalified Instrument after software upgrade and prepared quality documents. • Assisted subject matter expert and group lead with troubleshooting assay failures and technical complications. • Proficient in handling and preparation of serum, plasma, whole blood, and urine samples.
• Successfully performed Final Acceptance Testing (FAT) and pre-use qualification (QBU) of Early Engineering Prototype (Early EP) of assigned multifunctional high-throughput automated analyzer. • Ran 100+ automated assays daily on an average on the analyzer, analyzed and compiled test data. • Compiled, analyzed, and interpreted test results and prepared auditable quality documents pertaining to the FAT and qualification process, updated progress on test cases in Azure DevOps. • Troubleshot minor system issues daily, communicated major issues to system engineers and collaborated with them to investigate root cause and resolved them. • Performed preventative weekly and monthly maintenance of assigned instrument, updated, and maintained instrument logs. • Trained new project members on operation, maintenance, troubleshooting and safety of the analyzer, assisted newer members in Early EP qualification process. • Performed inventory management of samples and materials used for testing. • Responsible for restocking consumables and supplies in the assigned instrument. • Communicated progress with the team in daily and weekly meetings. • Labelled microcentrifuge tubes and aliquoted serum samples for immunoassay panel preparation for Limit of Detection, Limit of Blank, Limit of Quantitation, and Precision studies. • Assisted in filtering, pooling, spiking, and screening of bulk serum samples used for preparing panels.
• Investigated the feasibility of Ultrahigh-field (>7T) MRI guided Microwave Ablation of Liver tumors by computational FEM and simulations. • Conducted extensive research and performed comprehensive review of the literature survey and scholarly articles to test the overall hypothesis
MR Hardware and Simulations Lab • Modeled volume birdcage coils of resonant frequencies for standing to 1.5 T, 3T, 4.7T, 5.4T, and 7T using COMSOL Multiphysics software • Simulated the thermal changes induced by the birdcage coils in a human head phantom and spherical phantom with properties of biological tissues, after varying periods of exposure • Was responsible for comparative testing of softwares suitable for the research needs of the lab and facilitation of purchase of appropriate software from vendor Master's Thesis: Computational Modelling and Simulation of RF Induced Heating of Biological Tissue Link: https://search.lib.buffalo.edu/permalink/01SUNY_BUF/12pkqkt/cdi_proquest_journals_2453200002
Biomaterials and Regenerative Therapeutics Lab • Investigated the biochemical and mechanical properties of gelatin-based colloidal gels of varying concentrations to assess their biocompatibility as a suitable cell environment for human Mesenchymal stem cells. • Performed imaging of the sections of the gels by fluorescent microscopy. • Analyzed gel images to visualize the microstructures of the gel using ImageJ software. • Investigated the gelation kinetics of the gel particles by UV/Vis spectrophotometric analysis. • Quantified the protein internalization by the gels by determining the protein concentrations in the solvent. • Performed cell cultures to assess the viability of the Mesenchymal cells. • Conducted rheology studies of the gels to assess their mechanomorphology. • Organized, analyzed research data graphically and statistically and presented them to supervisor and colleagues at weekly laboratory meetings. • Reviewed relevant scientific literature and optimized experimental protocols. • Prepared and stocked all laboratory solutions and reagents efficiently. • Provided knowledge transition to new lab members; conducted training on project-specific practices.
• Assisted the team of researchers of the department to study and quantify the differential tissue expression of Aquaporin gene in samples of tissue from Clarius batrachus using qRT-PCR and DNA sequencing to investigate the role of Aquaporin in reproduction. • Kept accurate and up-to-date documentation of testing results.
Department of Microbiology, Viral research and diagnostics Lab •Managed and screened over 20 blood samples for Tuberculosis pathogen via PCR method each day. •Gained understanding of various instruments employed in the lab – Usage, maintenance, functioning and safety