Singapore
Final Year Project - Characterising the requirements for DENV-NS2B3 protein's interaction with and cleavage of hSTING
International summer research programme - Investigation of the potential regulation of Foxl2 by Isl1, Ascl1 and/or Pou3f4 through a super enhancer - 1 of 15 students worldwide selected by the Faculty of Biology, Technion, to work with their student researchers on a short-term research project
Determined dengue sNS1 structural changes when acidification of trans-golgi network (TGN) is inhibited - Expi293F expression system - Akta purification, SDS-PAGE, Western Blot, crosslinking - large-scale plasmid production and harvesting
- Completed high throughput screening of viral stability samples successfully using the TCID50 assay; proficient in other methods like QPA (incl. qPCR) - Gained experience in mRNA-lipid nanoparticle formulation, ribogreen assay and transfection - Actively participated in a cationic starch flocculation study using Roquette excipients -Supported Roquette in generating good amounts of high quality protein to support in-house projects by carrying out: Cell line expansion, maintenance & monitoring of metabolites; mAbs harvesting; protein purification via FPLC, HPLC (incl. ProA, AIEX, CIEX) - Proficient in cell culture/maintenance of selected mammalian cells