London Area, United Kingdom
R&D position: Contribution to the development of new cell lines - optimisation of stem cell culture conditions: passaging regime, media composition; metabolite analysis; imaging; suspension Erlenmeyer flask cell culture, stem cell differentiation; flow cytometry
R&D position: - Polymer synthesis, catalysis, development and validation of chemical innovations; usage of NMR, GPC, HPLC, universal testing machine (polymer tensile testing); - Research on biocomposites and adhesives - prototype development; - Implementation and execution of toxicity testing; - Research on liquid separation methods; - Account manager responsibilities; purchasing and negotiations
Checking chemical formulations of hospital CIVAS and chemotherapy orders – ratios, dilutions, formulations; final checking of the aseptically compounded products – syringes, IVI bags, CADD/Baxter devices; handling required documentation (including for controlled drugs - e.g. morphine). Handling chemotherapy drugs requiring special attention: azacitidine and various monoclonal antibodies.
06.06.2022 – 26.08.2022 - Nano-Safety Research Group, Heriot-Watt University, Edinburgh Prediction of toxicity of multi-component nanomaterials using in vitro and in chemico assays to reduce the need for animal testing – 12-week project funded by Animal Free Research UK Research activities included: 1. Assessment of reactivity of nanoparticles in an acellular environment. 2. Assessment of behaviour of nanoparticles in dispersion using dynamic light scattering. 3. Cell culture (THP-1 cell line) 4. Assessment of cytotoxicity of nanoparticles in order to select sub-lethal doses. 5. Assessment of reactivity of nanoparticles in a cellular environment. Used techniques: ferric reduction ability of serum assay, dynamic light scattering for the assessment of size, polydispersity, and zeta potential of nanoparticles, cell culture (THP-1 cell line) - culturing, sub-culturing, counting, plating, stimulation of differentiation; alamarBlue assay, glutathione depletion assays, adenylate kinase assay, lactate dehydrogenase assay, bicinchoninic acid assay;
An internship with the Cromar Future Group STEM education charity supporting local rural primary schools - development of chemistry study sessions, including detailed instructions for teachers, creation of educational images suitable for primary school pupils, and optimisation of the standard operating procedures for experiments and included hands-on activities.