London, England, United Kingdom
PhD student in the Ocular Cell and Gene Therapy Lab at King's College London. I use viral tracing to study retinal circuitry following cell replacement therapy and explore strategies to promote integration of transplanted photoreceptors.
I am working on characterising the restored retinal connectome following cell transplantation therapy and finding ways to enhance connectivity between transplanted photoreceptors and the host retinal circuitry.
I am part of the Wellcome Trust-funded MRes/PhD program Advanced Therapies for Regenerative Medicine. During the first year of the program (Master of Research), I completed three 12-week lab rotations: • Rotation project 1: Development of microfluidic chip-based photoreceptor cultures for screening and identification of neurite guidance cues • Rotation project 2: Corneal nanoinjection: a nanoneedle platform for gene editing in inherited corneal endothelial disorders • Rotation project 3: Annotating the human inner ear using single-cell RNA sequencing
AAV gene therapies for retinal diseases • molecular cloning, mammalian 2D cell culture maintenance, AAV-vector production and quality control, in vitro assays (lipofectamine transfection, RT-qPCR, Western blot)
• Thesis title: "In Vitro Modeling of Carboplatin Sensitivity in High-Grade Serous Ovarian Cancer Patient-Derived Organoids" • establishment and maintenance of 3D organoid culture, RT-qPCR, Western blot, immunofluorescence, single-cell RNA sequencing, drug-response assays, CRISPR-Cas9 mediated gene knockout
Maintaining and Reprogramming Cell Fates • Investigating the propagation of H3K4me3 in the absence of transcription in early Xenopus laevis embryos ‣ in vitro fertilization, embryo microinjection, PCR, qPCR, chromatin immunoprecipitation, molecular cloning, antibody validation
Chromosome Dynamics • Confocal microscopy, immunohistochemistry, quantification of mitotic chromosome segregation errors