Switzerland
Miriam is currently working on the production and characterization of AAVs for their use as delivery platforms in Cell and Gene Therapies (suspension cell culture, cell transfection, Western Blot, dPCR, ELISA). She has also experience working on genome integrity analysis and expression assays with AAVs (ddPCR, mRNA-based ddPCR, fluorescence-based assays, nanoDSF, cell cultures, primer design, DNA and RNA isolation, DLS). Previously, she worked on the synthesis and characterization of liposomes and cell-derived nanomembranes (cell cultures, thin-film hydration method, NTA) at CiQUS (Centre for Research in Biological Chemistry and Molecular Materials). Subsequent analysis were performed to evaluate their fusogenic capacity with biological membranes as well as their targeting efficacy (flow cytometry, microscopy). Also, she worked on RNA studies by using different molecular biology techniques (plasmid isolation, DNA sequencing, genetic and structural studies, primer design and inverse PCR mutagenesis) at Uppsala University. At the Cell and Molecular Studies Institute, she learned about how human samples are analysed through advanced sequencing tools in order to evaluate the predisposition to have a genetic disorder. She studied a MSc. in Biomedical Research, a BSc. in Biotechnology at Universidad de León and a Degree in Humanistic and Social Sciences from UFV (Madrid). She is fluent in Spanish (native), Galician (native) and English (advanced), and she has a basic knowledge of French.