Erembodegem, Flemish Region, Belgium
Waters Biosciences (formerly BD Biosciences) provides flow-cytometry and single-cell multiomics solutions with world-class support and continuous innovation. As pioneers of fluorescence activated cell sorting (FACS) technology, Waters Biosciences has provided flow cytometry expertise, world-class support, and continuous innovation for more than 50 years, enabling routine or complex FACS analysis to propel the goals of laboratories around the globe. Our work and our technology are currently changing how the world looks at an individual cell. Every day, we partner with our customers to help reveal the identity and function of billions of cells, one at a time. In addition, we also offer solutions for single-cell multiomics – a cutting-edge approach that integrates epigenomic, transcriptomic, proteomic, and other omics data to provide a comprehensive understanding of cellular biology with single-cell resolution. We continue to be at the forefront of discovery through innovations in informatics, genomics, proteomics, and image-based sorting, and we proudly offer an extensive portfolio of products to address our customers' needs. Waters is a global leader in life sciences and diagnostics, dedicated to accelerating the benefits of pioneering science through analytical technologies, informatics, and service. To learn more, visit waters.com. In my job as account manager I am following-up customers using flow cytometry and/or sorting or I want to meet those people who are planning to implement this cell analysis technique in their lab or institute. My customers are related to academic institutes, university hospitals, research institutes, CRO's, biotech/pharma companies, blood banks, clinical laboratories,... My aim is to provide my clients the most efficient and reliable solutions for their research questions or clinical tests. For more info you can visit our website: https://www.bdbiosciences.com/en-eu Questions or personal info/advice needed ? Contact me at [email protected]
Teaching/practical excercises of: Quality assurance Bacteriology Immunology Analytical chemistry
Interests and expertise: Molecular biology Cellular skin biology Oncology (melanoma) Immune-mediated skin disorders such as psoriasis Small RNA molecules (siRNA, miRNAs,...) Identification of novel drug targets Topical drug delivery Nanomedicines Elastic liposomes Cell culture of primary human skin cells 3D reconstructed skin models In vitro psoriasis models Transfections, electroporations and transductions (lentiviral vectors) of skin cells and cell lines Real-time quantitative RT-PCR, Western blots, ELISAs, immunocytochemistry, fluorescent microscopy, cell separation techniques (MACS/FACS), in situ hybridization, gene arrays,... Transferable and personal skills: Project management Lab & people management Stakeholder management Coaching and teaching Communicator and organisator Writing and presentation skills Dynamic and social Lifelong learning attitude This appointment includes the following duties: • Optimization of in vitro and ex vivo psoriasis models for studying the disease, and drug testing • Testing (topically or systemic) of novel therapeutics (siRNAs, small molecules) against psoriasis in ex vivo 3D reconstructed skin models • Involved in the identification of (possible) biomarkers to predict therapeutic response to biologicals in psoriasis patients • Involved in the establishment of a Biobank for immunologic skin disorders (in collaboration with Bimetra, UZ Ghent and implementation of SLIMS software) • Supervisor of lab technicians and PhD students (co-promotor) • Lab- and project management • Project writing and medical, technical writing (SOPs, Ethics Committee applications) • Peer review assessment of research work, and research project proposals of other groups • Budget calculations (pre-awards) and financial control of grants • Scientific reporting and publishing of data in peer-reviewed scientific journals • This work resulted in 26 peer-reviewed papers of which 3 as first and 3 as last author
This appointment included the following duties: • Introduction and application of RNA interference (transient or stable) to silence specific isoforms of the motor protein myosin Va in order to inhibit melanosome transport in human primary melanocytes. This work resulted in patent application PCT Pub. No. WO2007/068704 A1, published on the 21st of June, 2007 • Start-up of miRNA profiling of melanocytes followed by validation (qPCR, westerns, immunohistochemistry) and functional assays (transfections, luciferase assays) to identify target genes of aberrant miRNAs • Involved in the development of ultradeformable liposomes to deliver siRNA to the skin topically • Establishment of a 3D ex vivo reconstructed skin model (for use in depigmentation studies, topical application of molecules…) • Involved in the identification of genes or pathways playing a role in immune resistance in melanoma (FFPE material or blood samples) • Supervisor of PhD students and lab coordination (incl. biosafety) • Attending workshops and international meetings (+ presentation of own research work) • This work has led to 17 peer-reviewed papers of which 4 as first and one as last author
Appointed on a CERPER-grant via Pierre Fabre Laboratories (Toulouse, France). Development of non-viral carriers for the delivery of siRNA molecules, against Myosin Va exon F isoforms, in melanocytes for the treatment of hyperpigmentation of the skin.
• Monitoring of clinical trials and site management • Reviewing of Case Report Forms (CRFs) • Management of data of clinical research studies (ICH-GCP guidelines)