Beverly, Massachusetts, United States
• Next Generation Sequencing Workflows; Conducted DNA, cell-free DNA, and RNA extractions by various methods; DNA, cell-free DNA, and RNA quantification; library construction and operation of the Ilumina MiSeq and Illumina NextSeq Sequencers • Studied the reproducibility of company test(s) given RNA of varying ages and sources. • Built a research-use-only biobank including plasma, total white blood cells, extracted DNA, extract RNA and other sample types and designed a sample tracking methodology • Crafted and optimized different protocols to handle different sample types. • Collaborated with the Associate Director of Early Research to assess a novel technology for the company which employed a nonstandard samples type(s). The results were presented internally and the data set was shared with under-management • Contributing to the company’s understands of how immune cells impact cell-free sequencing results.
• Train new lab members in unique laboratory protocols • Coordinated experiments with visiting faculty and other laboratory b members • Oversee use of grant funds to run experiments • Acted as Lab Safety officer • Manage shipping of biological samples • Conduct experiments on Eosinophils to elucidate the Immuno-Biology of the human Eosinophils (and other innate immune cells) • Draw and purify the blood of consenting donors for experiments • Maintain IRB Compliance for blood draws • Manage Laboratory supplies, resources and service contracts • Coordinated patient sample acquisition with colleagues at other Clinical Trial sites
• Conduct experiments on Eosinophils to elucidate the Immuno-Biology of the human Eosinophils • Draw and purify the blood of consenting donors for experiments • Maintain IRB Compliance for blood draws • Manage Laboratory supplies, resources and service contracts • Coordinated patient sample acquisition with colleagues at other Clinical Trial sites • Processed samples from Mepolizumab EGPA Clinical Trial • Analyzed patient plasma for biomarkers • Aided RNs and Physicians in the execution of study protocols • Prepared patient binders for trial monitor visits
• Producing and studying nanogel surface functionality this includes: Synthesizing and purifying of nanogels precursors, assembling nanogels, analyzing physical and chemical characteristics of the nanogels • Participated in group meeting and joural clubs • Used group instruments such as DLS, NMR and separation columns • Use instruments to analyze compounds in each of the above steps • Document outcomes in the Laboratory in a Laboratory notebook • Designing and executing an independent senior thesis project (September 2014-May 2015).
• Worked with a team of scientists of numerous disciplines including biology, molecular biology and immunology • Used ELISA and Western blot to screening for Antibodies there are both Western and ELISA positive • Employed molecular cloning to produce various shuttle vectors • Performed Tissue Culture and Transfections of adherent and suspended cell lines