Cambridge, Massachusetts, United States
Norman Lab | Computational & Systems Biology Program
Bryson Lab | Department of Biological Engineering • Optimized a flow-sortable bi-functional assay for real-time quantification and visualization of phagosome acidification and proteolytic activity to identify genetic mechanisms of phagosomal environment regulation via a genome-wide CRISPR-KO screen. • Established and optimized murine macrophage in-house CRISPR screening and lentiviral transduction protocols for discovery of novel regulatory mechanisms of phagosome maturation. • Characterized phenotypic changes in murine macrophages as a result of perturbations to proteolytic activity and acidification. • Engineered a high-throughput method for FACS-based protease motif discovery using a modified Split-GFP circuit in E. coli and M. smegmatis to improve live macrophage M. tuberculosis death quantification assays.
Vance Lab | Department of Molecular and Cell Biology • Generated a list of orthologous viral effectors from diverse poxvirus genomes through the combined use of sequence and structural homology tools. • Employed a high-throughput luciferase reporter assay of NF-κB induction for systematic discovery of viral proteins capable of engaging effector-triggered immunity. • Characterized a novel eptesipox virus effector and harnessed co-immunoprecipitation to elucidate an alternate effector-triggered NF-κB induction pathway implicating canonical antiviral proteins TBK1 and N4BP1. • Furthered an in-house protocol for preparation of co-immunoprecipitation mass spectrometry samples from HEK293T cells for discovery of novel effector-triggered immunity proteins