Barcelona, Catalonia, Spain
👋 Hi and welcome to my LinkedIn profile! Thanks for showing interest in knowing a little bit more about myself! 💡 I have dedicated a big part of my professional life to research, gaining expertise in the analysis of proteomic data as well as using cutting-edge genome editing techniques such as CRISPR/Cas9. I define myself as a curious person and love discussing new ideas with my colleagues. My life as a researcher made me realize that the future is data driven, and that I definitely want to be part of it! 📈 Thanks to my background and career development I have been able to sharpen my analytical, critical thinking and storytelling skills. On top of that, further studying code and machine learning felt like a logical step to be able to deal with the ever increasing amounts of data and expand my repertoire of skills. The main Python libraries and tools I use are: NumPy, Pandas and Scikit-learn for operation, and Seaborn, Matplotlib, Plotly and Tableau for visualization. I also use API and web scraping for gathering data, and MySQL workbench and MongoDB as database management systems. 🚀 I would love to apply the knowledge I have been building up over the years on my next professional challenge :) 💻 Find my portfolio on my GitHub profile: https://github.com/mplanasgit?tab=stars 📖 You can also check my PhD thesis at: http://hdl.handle.net/10803/672165 📨 Please, feel free to contact me through private message or at: [email protected]
Team Lead for cross-functional projects: • Led a team of up to 6 researchers in daily task allocation, project timelines, and resource coordination, reporting to the Department Manager. • Managed workflows to ensure on-time delivery of molecular biology projects (e.g., qPCR and sequencing pipelines).
Full development of a highly multiplex qPCR oligonucleotide assay for the detection of pathogens and AMR genes from human blood samples
Full development and validation of Methods for: • Detecting and quantifying specific interleukins in humans and non-human primates. • Detecting and titrating anti-drug and anti-PEG antibodies. • Determining the potency of enzymatic drugs.
I worked on the interaction between crops and the quarantine plant pathogen Ralstonia solanacearum to develop new strategies to fight bacterial invasions in the field. My research focused on 4 main topics/projects that involved: • Quantitative proteomics data analysis (using MaxQuant Perseus and R) • Bioinformatics and functional analysis of tomato proteases (P69 subtilases) using CRISPR/Cas9. • Plant grafting and histology (confocal microscopy and chemiluminescence imaging). • Investigation of bacterial virulence regulators during plant infection. Main outcomes: 5 papers in peer-reviewed journals (2 first-author and 3 co-authored), participation in 6 international conferences (3 oral presentations, 3 posters), and 3 internships in leader laboratories from the Oxford University (UK), Hohenheim University (Germany) and INRA-Avignon (France), which provided me the expertise in cutting edge techonologies for the characterization of Protein activity, Protein production and purification, and CRISPR/Cas9 mutagenesis. Acquired technical expertise: • Data analysis of quantitative proteomics data. • Production and purification of proteins from plants. • CRISPR/Cas9 mutagenesis and plant transformation. • Manipulation and pathogenicity assays with quarantine pathogens. • Confocal microscopy and chemiluminescence imaging. • Bioinformatics, Proteomics and Functional Genomic tools. Additional expertise: • Management of 4 parallel projects. • Supervision and training of B.Sc., M.Sc. and junior Ph.D. students. • Scientific writing and communication skills.
Production and purification of tomato P69 proteases for their application on the bioassay Proteomic identification of cleavage sites (PICS). Supervisors: Prof. Dr. Andreas Schaller and Dr. Annick Stintzi
Protoplast transformation and generation of CRISPR/Cas9 transgenic tomato lines for the deletion of P69 proteases. Supervisor: Dr. Marianne Mazier