Lukas Tombor

Assay Tech Expert | single cell sequencing and spatial transcriptomics specialist

Berlin, Berlin, Germany

About

Thrilled to be part of the forefront of new biotechnological advances shaping our understanding of diseases and driving progress in personalized medicine. As a dedicated team player with a biology PhD background and extended experience in bioinformatics, I excel in posing intriguing questions and drawing comprehensive conclusions. My passion for interdisciplinary problem-solving thrives in collaborative environments, where I enjoy communicating scientific ideas to inspire others. The future of medical advancements requires technological progress and exceptional collaborative efforts from interdisciplinary teams. This will be especially true when harnessing the full potential of single-cell sequencing techniques to treat patients with greater precision in the future. As an all-rounder, I transitioned from a complete wet lab expertise background to a computational and methodological focus. My journey into coding started at the beginning of my PhD when analyzing single-cell sequencing datasets ignited a deep fascination for the logical thinking and learning-by-doing nature of programming. Furthering my commitment to data science, I successfully completed a medical data science program, enhancing my proficiency in machine learning and data science algorithms with direct applications in single-cell sequencing. In my current role as Assay Technology Expert at Bayer, I am genuinely excited to contribute to a vision of improving patient care by integrating exciting new technologies. Eager to play a vital role in advancing our understanding of diseases and applying cutting-edge biotechnologies.

Experience

  • Assay Technology Expert at Bayer
    Jan 2024 - Present · 2 yrs 6 mos

  • Johann Wolfgang Goethe-Universität Frankfurt am Main (Frankfurt am Main, Hesse, Germany)
    • Postdoctoral Researcher
      Oct 2022 - Dec 2023 · 1 yr 3 mos

      Head of the endothelial heterogeneity team Research on how the microenvironment can change cell fate decisions with the goal of developing future therapeutic options in patients with heart failure. Established spatial transcriptomic sequencing in the lab to study spatiotemporal patterns of endothelial cell states. I developed a computational approach to optimize cytokine treatments in cell culture with the goal of maximizing cell transitions. Implementing bioinformatic pipelines in R and Python FACS-based T-cell assay development Supervisor of PhD students (2 wet lab, 1 dry lab student) and technical assistance personal Scientific collaboration partner and technology expert within the National Excellence Cluster for cardiovascular and lung research Primary statistician in clinical study with cardiac patients Co-author of Science and Nature Cardiovascular Research Articles on Cardiovascular Ageing and single-cell heterogeneity (https://www.science.org/doi/10.1126/science.ade4961)

    • PHD Researcher
      Apr 2018 - Oct 2022 · 4 yrs 7 mos

      Endothelial heterogeneity in cardiovascular repair Primary focus on single-cell sequencing analysis and validation in cardiac endothelial cells. Establishing single cell wet and dry lab workflows in models of cardiovascular aging and disease Instructor for bioinformatic analysis in R. Facility work for bioinformatic analysis. Cell culture and molecular biology assays. Confocal microscopy and FACS Conducted, organized, and supervised animal experiments for cardiovascular research. The organizer of PhD summer schools and lab retreats. Supervised diploma and internship students. 50% computational, 50% wet lab work.

  • Dipolma/Master student at Institute of Molecular Biotechnology, Vienna, Austria
    Mar 2016 - Dec 2017 · 1 yr 10 mos

    Master thesis under supervision of Prof. Dr. Josef Penninger. Involved in a project in direct reprogramming of fibroblasts into cardiomyocyte-like cells. Gained experience in tissue culture including knockdown and knockout (CRISPR/Cas9) experiments in mouse primary cells, Working and targeting ESCs and S2 laboratory viral vectors (lentivirus, retrovirus). In vivo mice models. Trained on many conventional wet-lab applications such as cell imaging, cloning, flow cytometry, histological sectioning, RT-PCR and PCR, Southern blot, Western Blot.

  • Student Research Assistant at University of Vienna
    Oct 2015 - Jan 2016 · 4 mos

    Involved in stress marker identification in Cavia procellus. Design of qPCR and dPCR experiments Isolation of DNA and quantification from biological samples Tutoring in a Master’s course on Molecular Biology

  • Bachelor Student at Veterinary University of Vienna
    Feb 2015 - Sep 2015 · 8 mos

    Worked on FGF23 in the aorta and it’s effects on nitric oxide production. Animal and primary cell culture experiments Nitric oxide measurement (Cooperation with LBI Vienna)