New London County, Connecticut, United States
Characterization of biotherapeutic proteins using mass spectrometry specifically looking at post-translational modifications (i.e. glycosylation, disulfides, oxidation, deamidation phosphorylation, glycation, etc.) via intact mass or peptide mapping LC-MS. Also, perform N-linked and O-linked glycan profiling.
Characterization of proteins using mass spectrometry specifically looking at post-translational modifications (i.e. glycosylation, disulfides, oxidation, deamidation phosphorylation, glycation, etc.) via intact mass or peptide mapping LC-MS. Various proteins characterized are AAVs, antibodies, glycoproteins, and more. Also, routinely perform N-linked and O-linked glycan profiling as well as lipid profiling by LC-MS. Responsible for writing technical reports, presenting to clients, and mentoring others.
Responsible for characterization of soluble proteins, GPCRs, and solute transfer proteins by mass spectrometry for structure determination by x-ray crystallography, cryoEM, NMR, and for screening of compounds by various assays including SPR and ASMS. Specifically studied post-translational modifications (i.e. phosphorylation, glycosylation, disulfides, etc.), chemical modifications by targeted probes and other covalent binders, as well as lipids bound to various proteins. Applied HDX/MS to study conformation dynamics of a glycosylated protein with many disulfides and its protection by a cyclic peptide. Purified soluble recombinant proteins. Responsible for writing summaries, presenting at project and department meetings, and mentoring others.