St Louis, Missouri, United States
Undergraduate student studying Biology at Saint Louis University Undergraduate researcher working in regenerative medicine & longevity.
Mokalled Laboratory: This group focuses on characterizing the different cell types within the regenerating Zebrafish spinal cord, and uncovering the mechanisms that lead to successful spinal cord regeneration in Zebrafish. My project is focused on characterizing astrocytes and their contributions to spinal cord regeneration through snRNA-seq analysis, HCR FISH, IHC, and electrophysiology.
Chan Laboratory Investigating the mechanisms by which SerpinE2 mediates 4T1-H2.35 cell competition, and the potential roles of TNFa and IFNy in this process. - Generated lentivirus constructs for transduction into 4T1 and H2.35 Murine cell lines - Utilized Immunostaining and Fluorescence Microscopy to determine outcomes of 4T1-H2.35 cell competition. - Established a protocol for utilizing a QuPath machine learning classifier to distinguish 4T1 cells from H2.35 cells in CoCulture
Lemaire Laboratory Establishing a CRISPR/Cas12 system to generate knock out lines in F0 Ciona Intestinalis - Generated lineage specific Cas9 constructs using OSO-PCR - Electroporated Cas9 and sgRNA constructs into Ciona Intestinalis zygotes Investigating allele expression bias of FoxF in Ciona Savigni-Intestinalis hybrid larvae - Performed 3D image analysis of Ciona larvae to determine FoxF expression in heart progenitor cells Transcriptional mapping of neural crest progenitor cells across larval development in Ciona Intestinalis - Generated reporter constructs for in vivo expression - Perfoemed image analysis of stained Ciona larvae to determine reporter construct expression
LongX is a non-profit devoted to improving education and access to longevity science for global student body. - Developed 2 course chapters: “Ethics of longevity” & “How we talk about longevity” - Presented live to a cohort of 20 fellows. - Moderated in-depth discussions between fellows on regulatory & societal impact.
Chaney Laboratory Utilizing a novel antibody-radioisotope conjugate to track peripheral myeloid cell infiltration into the central nervous system as a prognostic marker of neurodegeneration - Collected mouse brain and spleen tissue samples via cryosectioning - Conjugated EDTA and EGTA to a novel anti-peripheral myeloid cell marker - Radiolabeled a novel chilator-antibody conjugate with Carbon-14 and Fluorine-18 - Utilized autoradiography and a mouse plasma stability assay to determine metabolism and localization of a novel chilator-antibody conjugate