Vancouver, British Columbia, Canada
· Work in a team from two to six people to ensure an efficient process of taking orders and crafting beverages or handling food orders while maintaining sufficient stock and customer connections
· Craft beverages according to standard recipes to produce drinks of the highest quality in a fast-paced environment
· Multitask by tending to the customers, crafting drinks and handling food orders at the same time
· Receive, sort and prepare beverages for the barista during peaks to increase efficiency
· Clean and restock to ensure safety and ability to handle another rush
· Maintain a connection with customers as customer connection is the highest priority
· Clean and maintain the equipment used in store
· Calculate and distribute tips based on hours worked for each partner
· Training new partners from scratch as a Barista Trainer
· Part of the JHSC (Joint Health and Safety Committee) team
· Performed protein purification using complete His and Ni-NTA gravity columns, DEAE and size-exclusion chromatography columns using the AKTA · Performed antibody purification using Protein A agarose beads · Concentrated purified proteins using protein concentrators · Performed bio-layer interferometry using the Octet to determine dissociation constant of antibodies to antigens · Performed cell-based assays using flow cytometry to determine binding probability of antibody to antigen · Prepared and executed PCR and mutagenesis reactions, gel extraction, and subsequently Gibson ligation or T4 ligation · Transformed plasmids into competent cells to be plated on LB plates with specific antibodies · Performed minipreps and midipreps · Prepared miniprep samples to be sent off for sequencing · Prepared titration and neutralization assays to determine viability of pseudo viruses and the ability of various antibodies or serums to neutralize specific pseudo viruses measured with RLU · Maintained mammalian cell lines and seeded them for subsequent usage in various assays · Synthesized various antigens and antibodies using transient transfection of suspension mammalian cell lines and purified them · Quantified proteins using Rapid Gold BCA assays · Performed SDS-PAGE and Native PAGE to determine the purity of the protein of interest or to determine the estimated size of the protein of interest · Maintained a detailed lab notebook detailing the various steps took and the reagents used in each experiment · Followed recipes to make the buffers needed · Autoclaved large batches of biohazardous waste and lab equipment
· Performed protein purification using a sonicator, Ni-NTA gravity columns and dialysis · Concentrated purified proteins using protein concentrators · Ran large batches of samples through the SDS-PAGE to determine if the purification was successful · Transformed plasmids into competent cells and plated them on LB plates with specific antibodies · Inoculated small and large cultures with the transformed cells · Pelleted the large cultures and froze them for later use in protein purification · Prepared different types of media for small and large cultures and for agar plates · Kept track of the inventory and updated an order sheet whenever anything is running low · Made reagents according to the recipes and brought them up to the required pH · Kept track of the different steps performed in a lab notebook · Autoclaved various items: tips, flasks, buffers, biohazardous waste, media