Leah Zagore, PhD

RNA Biologist | Bioinformatician

Cleveland, Ohio, United States

About

PhD scientist with 15+ years of experience generating, integrating and interpreting multiomic data to drive discovery in translational research. Deep expertise in experimental design, preparation, and bioinformatic analysis of next-generation sequencing datasets, including single-cell RNA-seq, spatial transcriptomics, ribosome profiling, CLIP-seq, and RNA-seq. Proven ability to manage multiple independent projects, troubleshoot complex workflows, and communicate results across diverse research teams. Strong publication record with 13 peer-reviewed articles (4 first-author), including in Nature and Molecular Cell. Proficient with Python, R, Linux command-line tools and writing shell scripts. Detail-oriented, collaborative, quick to adapt to emerging scientific tools and analytical platforms, and always eager to learn.

Experience

  • Case Western Reserve University (3 yrs 9 mos)
    • Core Manager, Advanced RNA Profiling Core
      Feb 2026 - Present · 6 mos

    • Research Associate - Specialized Technical Skills
      Nov 2022 - Feb 2026 · 3 yrs 4 mos

      Spatial Platform Lead - Applied Functional Genomics Core: Consultant and project lead for spatial transcriptomics services and collaborative research support. -Led scRNA-seq and spatial transcriptomic analyses across multiple disease-focused projects (COVID, cardiovascular disease, colorectal cancer, oral cancer, Sezary syndrome). -Designed and built pipelines for end-to-end bioinformatics: raw data processing, QC, normalization, dimensionality reduction, clustering, cell-type annotation, differential expression, pathway/Gene Ontology analysis, ligand–receptor inference, RNA trajectory/velocity, and PPI network modeling. -Leveraged expertise in molecular biology to integrate multiomic datasets (bulk, single-cell, spatial RNA-seq, proteomics, imaging) to uncover novel disease mechanisms and biomarkers. -Managed 14+ spatial and single-cell service core projects over 3 years, responsible for client consultations, wet lab execution, and bioinformatic analysis. -Provided consultative expertise in spatial, single-cell, and RNA-seq study design and analysis for grant submissions and clinical trial protocols. -Spearheaded adoption of new spatial platforms and organized campus-wide educational seminars. -Mentored PhD students in experimental design, data analysis, and scientific communication.

  • Postdoctoral Research Scientist at Center for RNA Science and Therapeutics
    Jul 2020 - Nov 2022 · 2 yrs 5 mos

    Performed research investigating novel RBPs with essential roles in early CD4+ T-cell activation | Trained and managed projects for undergraduate students and research assistants | Researched and established specialized protocols for new laboratory techniques

  • Case Western Reserve University (8 yrs 11 mos)
    • NIH NRSA Predoctoral Fellow (T32)
      Sep 2014 - Sep 2019 · 5 yrs 1 mo

      Advisor: Dr. Donny Licatalosi Thesis Title: The molecular function of the RNA binding protein DAZL in male germ cell survival. -Served as technical and analytical consultant to external labs, supporting sequencing library prep and bioinformatic analysis (RNA-seq, PolyA-seq, ribosome profiling, iCLIP, polyA tail profiling). -Investigated RNA-protein interactions using an integrated approach (transcriptomics, proteomics, cell culture models, animal models) to define cell-specific regulatory programs important for male fertility. -Contributed to the development of novel technologies: dual-fluorescent flow cytometry approach for germ cell isolation; in vivo measurements of RNA-protein binding kinetics. -Acted as lab manager and mentor; trained students and coordinated projects (2014–2022). -Implemented new protocols in the lab including primary T-cell isolation (MACS), CRISPR genome editing, ribosome profiling, RBP proteomics, and stable cell line generation.

    • Research Assistant II
      Nov 2013 - Sep 2014 · 11 mos

      Performed research investigating the role of RNA binding proteins in reproductive health and fertility. Acted as a consultant for outside laboratories attempting HITS-CLIP and prepared several HITS-CLIP libraries for collaborators. Worked as laboratory manager in Dr. Licatalosi’s lab - responsible for training graduate and undergraduate students, ordering reagents and inventory management.

    • Research Assistant I
      Nov 2010 - Oct 2013 · 3 yrs

  • Ballroom Dance Instructor at La Danse Cleveland
    Oct 2012 - Sep 2014 · 2 yrs

    Led individual and group ballroom dance lessons in various dance styles following DVIDA curriculum. Assisted in special event planning and execution.