San Francisco Bay Area
Managerial Role: Responsible for the oversight of lab maintenance and organization, team research projects and individual productivity goals, and the safety and training requirements of all lab members • Head technician leading a team of research assistants • Project manager and liaison for international and domestic research collaborations • Meeting coordinator for laboratory research and project meetings • Creator and editor of medical, biosafety, human subjects, and animal protocols • Compliance coordinator for Santa Clara County and Stanford University inspections • Primary contact for sales representatives • Curator of 30,000+ vials of clinical specimens Laboratory Responsibilities: Consistent multitasking, prioritizing, and problem solving abilities required to fulfill both managerial and scientific duties • Teach and train clinical and postdoctoral fellows, graduate students, and interns in a teamwork setting • Stain and stimulate cells for hi-dimensional surface and intracellular flow cytometry and phosphoflow • Utilize cyTOF mass cytometry to generate multiparameter single cell data analyses • Design and optimize assays for cytokine detection by ELISA for patient diagnoses and treatments • Isolate and cryopreserve peripheral blood mononuclear cells by density gradient centrifugation • Perform magnetic cell separation and cell sorting techniques to segregate and enrich cell subsets • Purify and conjugate monoclonal, polyclonal, and metal-labeled antibodies • Isolate genomic DNA and RNA for quantitative real time PCR • Grow and culture human and murine cell lines • Enroll and consent participants for human research studies • Set up and maintain equipment, service, and repair contracts • Generate procurement requisitions • Create, edit, and submit federal grant applications
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• Collaborated with a research team conducting a Phase II Clinical Trial on adjuvanted influenza vaccine and immune responses in the elderly • Isolated, stimulated, and cryopreserved human peripheral blood mononuclear cells • Reviewed and edited SOPs
• Isolated and cryopreserved peripheral blood mononuclear cells by density gradient centrifugation • Designed and optimized assays for cytokine detection by ELISA for patient diagnoses and treatments • Stained and stimulated cells for 3-4 color flow cytometry analyses • Assisted with animal husbandry and experimental mouse model dissections • Isolated genomic DNA and RNA for quantitative real time PCR • Grew and cultured human and murine cell lines • Created, edited, and submitted federal grant applications and SOPs
• Assisted with federal grant applications regarding asthma immunology, clinical immunodeficiencies, infectious diseases, and influenza • Provided, organized, and catalogued more than 15,000 scientific research publications • Updated current protocol journals and created bibliographies using Endnotes Program