Seattle, Washington, United States
1+ year experience leading supply chain operations at the Singapore Armed Forces 4 years laboratory experience at the University of Washington, Department of Bioengineering: - Developed a paper-based hydrogen fuel cell for point-of-care diagnostics - Developed a rapid and sequence specific nucleic acid capture method to extract HIV DNA from human peripheral blood mono-nuclear cells - Designed and cloned fluorescent proteins into viral vectors containing nuclear localization signals to monitor gene expression dynamics at the single cell level using live-cell imaging - Developed a sequence specific, nucleic acid based glass slide array to immobilize endonuclease modified chromatin for epigenetic studies
Taught principles of biological circuit design using Tellurium, a Python based integrated development environment, to simulate and study biological circuits
Developed and optimized a sequence specific nucleic acid capture platform to purify HIV DNA from human genomic DNA in PBMC lysates limited resource settings to improve the specificity and sensitivity of HIV Drug Resistance Test Kit (OLA-Simple). • Designed DNA purification platform using microcentrifuge filters packed with streptavidin resins • Designed probes and primers for HIV DNA on Benchling • Evaluate capture efficiency of low DNA copies using qPCR • Analyze product amplification specificity using gel electrophoresis • Synthesize short HIV RNA templates using T7 polymerase • Synthesize short HIV cDNA templates using reverse transcriptase Mentors: Dr. Barry Lutz, Nuttada Panpradist, Amy Oreskovic
Engineered DNA immobilizing glass slide via UV crosslinked probes and demonstrated functionality using a mini ELISA. Histone markers on captured DNA will be analyzed using TIRF microscopy to better understand epigenetic regulation. • Cloned fluorescent reporter into gene construct containing nuclear localization signal • Viral packaging and transduction of gene construct into Jurkat cell line • Verification of transduction via flow cytometry • Preparation of P2C2 histological sections using formaldehyde fixation • Preparation of P2C2 genomic DNA using endonuclease and exonucleases • Preparation of UV crosslinked DNA probes on a glass slide and experimental validation of probe functionality Mentors: Dr. Hao Yuan Kueh, Sam Nguyen, Matthew Wither
Led a team of soldiers to perform maintenance and loaning of military equipment to training units. Collaborated with officers regarding training plans to coordinate equipment allocation.