Waynesboro, Pennsylvania, United States
Experienced and highly accomplished immunologist with 10+ years of in vitro and in vivo experience of human and murine models of autoimmunity and cancer. With demonstrated exceptional organizational, leadership and communication skills, I am highly motivated to apply my technical and professional skills to advance translational cell-based therapies in a team-oriented and collaborative environment. Areas of expertise: Cell therapy, immune tolerance, T cell biology, CAR-T cell engineering, myeloid cell biology, mouse models of diabetes and cancer, autoimmunity, tumor immunology, immune checkpoints, preclinical drug development, cell based in vitro assays, flow cytometry.
Technical Skills o Cell-based assays: spectral flow cytometry, cell cycle modulation, high multiparameter panel design and optimization, spectral and conventional flow cytometry, CAR degradation assays, cytokine production assays, multiplex ELISA, xCelligence killing assays, cell-based assay development and optimization. o Cell culture: primary human T cell culture, stable and transient transfection, lentiviral production, cell transduction, CAR-T cell generation. o Imaging: spinning disc confocal imaging, quantification, and data analysis o Molecular biology techniques: lentivirus construct design and optimization, PCR, bacterial transformation, cloning, ddPCR, qPCR.
● Technical Skills: o Cellular based assays: spectral and conventional flow cytometry analysis with spectral unmixing or compensation, phospho-flow cytometry, caspase-1 FAM/FLICA flow cytometry flow assisted cell sorting, ELISA, multiplex ELISA o Imaging: live cell imaging using 2-photon microscopy on explanted islets o Cell culture: mammalian cell culture, primary T cell culture, primary bone-marrow derived macrophages and dendritic cell culture, immortalized tumor cell lines, islet culture o In vivo mouse model skills: basic mouse handing and injections, dissection, tissue harvest, pancreatic islet isolation, mouse husbandry and colony maintenance o Molecular biology techniques: PCR, reverse transcriptase PCR, quantitative PCR
Technical Skills: • Cellular based assays: conventional flow cytometry analysis with compensation • Cell culture: mammalian cell culture, immortalized tumor cell lines • In vivo mouse model skills: basic mouse handing and injections, dissection, tissue harvest, mouse husbandry and colony maintenance, mouse orthotopic tumor implantation surgery, mouse hemi-splenectomy tumor implantation surgery • Histology: cryostat microtome sectioning, immunohistochemistry staining on FFPE sections, immunofluorescent staining on frozen tissue sections, hematoxylin/eosin tissue staining • Areas of expertise: T cell biology, Mouse models of cancer, Autoimmunity, Tumor immunology, Immune checkpoints