Charlottesville, Virginia, United States
*Be responsible for project management, lead and contribute to development of genetically encoded bioluminescent and fluorescent biosensors for imaging biological processes and answering biological questions. *Individually carry out experiments such as plasmid design, PCR, DNA separation, gel electrophoresis, protein engineering, directed evolution, site-directed mutagenesis, protein expression and purification in bacterial and cell culture, SDS-PAGE, FPLC, protein and enzyme assay, mammalian cell culture, live cell fluorescence imaging and bioluminescence imaging, *Optimize and document procedures and methodologies for assessment of evaluation of probes in enzyme assays, live cell assays and animal experiments. *Plan experiments and conduct testing of large number of samples in plate-reader based assays. *With limited supervision, design and implement protocols, as well as troubleshoot assays and experiments independently. *Evaluate, analyze and compile experimental data. *Be responsible for ordering supplies, and maintaining equipment and inventories. *Participate in weekly group meeting and present data and report. *Track on literature and state-of-the art techniques related to project. *Prepare scientific manuscripts and be responsible for the submission and communication with journal. *Present at professional meetings, seminars and informational sessions. *Work under EHS Chemical and Laboratory Safety Group regulation, Institutional Biosafety Committee (IBC) regulation and Animal Care and Use Committee (ACUC) regulation. *Other duties assigned by PI.
In this position, I: • Supervised and instructed total 96 undergraduate students in two semesters • Facilitated students studying of chemistry concepts and experimental procedures through a guided inquiry approach in workshops • Provided feedback on lab technique, ensured proper operation of equipment, encouraged student-student interaction in laboratory • Organized students’ presentation and facilitated questions and discussion following presentation • Graded students’ homework, presentations and discussions, and returned graded materials to students in a timely basis • Held office hours to address concerns and questions from all the students attending this course • Attended weekly TA-meetings • Maintained a safe working environment in the laboratory