Cambridge, England, United Kingdom
With a BSc in Biochemistry from the University of Leeds and two years of experience in pharmaceutical settings, I have gained extensive experience in protein purification, molecular biology, and analytical techniques. From my laboratory experiences in industry, I gained experience in: - Gel Electrophoresis - AKTA operation and chromatography - UF/DF - Tecan 200 & Freedom EVO - Labchip - UPLC/HPLC ( SEC, ProA, reverse phase, ion exchange) - ELISA During my dissertation project at the University of Leeds, I applied myself to molecular biology and protein engineering to create structural mutants of an S.aureus Elongation factor G construct. From my experience in a research lab I developed skills in : - PCR - DNA extraction - E.Coli cell culture - Aseptic technique - Multistage protein purification - NMR. Overall, I am a creative and analytically-minded individual with a continuous desire to learn and develop new skills. With my diligent approach and strong problem-solving abilities, I thrive in collaborative environments and am eager to tackle new challenges.
Independently planned and executed chromatography and UF/DF experiments and follow-up analysis to characterise the downstream process for a therapeutic fragment antibody. Purified over 40 samples using automated liquid handling robotics, guaranteeing clear and high-quality results of HPLC analysis of eluate samples, free of host cell proteins and contaminants, improving the reliability of experimental results. Maintained a safe and organised lab environment adhering to health and safety standards, COSHH regulations and standard operating procedures Software: AKTA, Empower, Tecan Freedom EVOware, Microsoft Office suite, Go-silico
Undertaking a final year research project creating structural mutants of an S. Aureus Elongation Factor G construct for NMR analysis. Laboratory techniques: QuickChange PCR, E.Coli cell culture, transformation, plasmid miniprep extraction, protein purification using Ni-NTA affinity and size exclusion chromatography, SDS-PAGE, Induction, sequence analysis, NMR.
Collaborated working on various customer projects, operating downstream processing equipment to achieve GMP standard protein purification and processing. Laboratory skills: Protein purification synphonx and AKTA models at a variety of scales, Solo VPE, Nanodrop, Buffer Preparation, SDS-PAGE. worked within a team to develop a high through put assay to identify ideal refolding conditions of inclusion bodies found within E.coli cells lines. Skills: Tecan liquid handling automation, LabCHIP, data analysis,