Hisar, Haryana, India
Hands-on Technique 1. Virology. Virus Isolation and Propagation (BSL-3 Level), like SARS-CoV-2, Bovine and Equine coronaviruses, Equine Herpes Viruses 2 and 5, Rotavirus, Japanese encephalitis, West Nile virus. Isolation in Animal Models. Multiplicity of Infection (MOI) Optimization. 2. Mammalian Cell culture. Cell Culture-Based Virus Propagation Techniques, Cell Viability and Cytotoxicity Assays, Plaque assay, TCID₅₀, Focus forming assay, Imaging and Cell Analysis Techniques, Growth Kinetics, Cryopreservation, Worked on cell lines like RK-13, NLBK, MA104, and Vero cell lines, CHO, HEK293. 3. Genome Editing: High-Efficiency Gene Delivery Technologies, Design the Guide RNA, CRISPR direct, Benchling, Crispor, Designing Genetic Cassettes for CRISPR/Cas9-Based Vaccine Development, Electroporation. 4. Molecular Biology: PCR, cDNA synthesis, RT-PCR Assays, Primer synthesis, Isolation of genomic DNA from tissue and whole blood, DNA isolation by automatic kits, Gel electrophoresis, Spectrophotometric estimation of isolated DNA, RNA-PAGE. 5. Sample Processing: Preparation of viral transport medium, Sample lodgment, assembly, and Storage. 6. Bioinformatics: Worked on software like Clustal W, Clustal Omega, ExPASy, OligoCalc, Swissport, NCBI, BLAST, Mega X, and BioEdit software. 7. Immunology: ELISA, Immunodiffusion, Immunoelectrophoretic, handled various therapeutic kits. 8. Administrative skills: GeM portal, ERP, E office.
Design and execute experiments, perform RT-PCR, real-time PCR, and sequencing, Phylogenetic and epidemiological analysis, Analyze and interpret genomic data