Basel, Basel, Switzerland
• >15 years in pharma R&D (biologics / DSP team leader @NIBR) • fields of expertise: process development (expression strategies & DSP), protein engineering, protein folding, biophysics, optical spectroscopy, bioinformatics, bioanalytics, gene design • main responsibilities: DSP team leader (downstream processing of microbial, mammalian & insect cell culture products: protein purification, refolding, modification, and analytics); project management (therapeutic protein project team leader) • For mastered techniques see Experience section. Key strengths: analytical thinking, creative problem solving and perseverance.
Protein science & biotechnology expert @NIBR Biologics Center (NBC), leading a protein biochemistry and purification lab. Member of several biologics project teams @NIBR, antigen/therapeutic protein strategy (protein design and engineering, expression and antibody generation strategy, etc.). Downstream processing (DSP) of mammalian, insect and yeast cell culture supernatants as well as of E.coli and insect cell pellets. DS Process development using design of experiments (DoE). Protein refolding from the sub-µg to the double-digit gram scale. Refolding and purification process development with focus on scalability. Protein characterization/analytics. Chemical modification (biotinylation, fluorescence labeling, PEGylation, ...). Construct design & protein engineering. People management, lab management, budget management, resource allocation. Expertise: biologics / biotherapeutics, protein refolding from inclusion bodies (interleukins, cystine-knot and other growth factors, other cytokines / chemokines, receptor ECD's, uncharacterized proteins (orf's)...) and protein purification (peptides/small proteins/IgG's/virus-like particles (VLP's); broad range of chromatographic methods; ultra- and diafiltration; lyophilization); formulation; endotoxin removal; chemical modification of proteins; hapten conjugation; analytical and preparative RP-HPLC of proteins; lyophilization (freeze-drying); biophysical chemistry; protein folding; optical spectroscopy of proteins (UV absorption, fluorescence, CD; derivative); dynamic light scattering (DLS); advanced data analysis; bioinformatics: sequence analysis, protein design/engineering and gene design and optimization for efficient recombinant protein expression (soluble and membrane proteins; grafting/chimeric proteins). Software: Wolfram Mathematica, Jalview, Pymol, MOE, AlphaFold, Thermo/Dionex Chromeleon, GE UniCorn, Agilent ChemStation, Origin, Microsoft Office, LaTeX Languages: English, German
Single-molecule FRET analysis applied to problems of protein aggregation and chaperon-assisted protein folding in the group of Ben Schuler.