London, England, United Kingdom
I have always been interested in all things health and disease, from how certain genes predispose individuals to develop certain conditions to disease management and treatment. These phenomena have prompted me to undertake a degree in Biomedical Science to learn about the body and its underlying unique features to combat disease. With the knowledge I gain in this course, and throughout my involvement in this field, I hope to make a positive impact in the health sector in the future.
I researched the effect of bone morphogenetic protein 6 (BMP6) on the terminal differentiation of keratinocytes using rat epidermal keratinocytes as an in vitro model. I performed cell culturing using aspectic techniques, and western blotting and immunofluorescence to assess the effect of BMP6 expression on terminal differentation markers: loricrin and desmoplakin 1 and 2. Additionally, I used imaging software such as 'ImageJ' to analyse the western blots and immunofluorescence images to help me critically analyse the data and formulate conclusions about the effect of BMP6 on differentiation. This evidence helps our understanding of BMP signalling, hence proving it to be a promising therapeutic factor in atopic dermatitis treatment.
I contributed to a project that used in-situ hybridisation to analyse the effects of different drug treatments, which were Crispr-Cas9 injections against different cardiac markers, and analyse the effect on different parts of the retinoic pathway in zebrafish embryo cardiac development. Specifically, I performed an in-situ hybridisation to understand the effect of Crispr-Cas9 injections against the cardiac markers: tbx1, Cyp26B1 and both tbx1 and Cyp26B1. I used the digital imaging software ‘uEye’ to visualise the effects of the different drug treatments on the staining of the developing hearts of the zebrafish embryos.
I received and checked invoices, assisted customers, organised prescriptions and restocked shelves with medication and other items.