Cambridge, England, United Kingdom
The work in the lab of Timothy Jenkins and Andreas Laustsen-Kiel was a collaborative investigation at the interface of de-novo protein design and experimental validation of high scoring hits.
Development of a reliable ultra-high throughput screening platform for PET hydrolysing enzymes utilising droplet microfluidics for compartmentalisation of reactions, and FACS for screening.
Thanks to the EMBO scientific exchange grant I was able to spend 4 months of my PhD in Michael Krogh Jensen's lab at DTU's Center for Biosustainability. The main goal was to expand the selection of host organisms compatible with the screening platform for enzymatic PET hydrolysis, which was developed in the context of my PhD, by the eukaryote S. cerevisiae. One focus was the development of a reliable, S. cerevisiae-based, whole cell biosensor.
After having spent 1 year in the lab of Pam Silver at HMS during my master's studies at the University of Tübingen, I returned to the Silver lab to continue work on ongoing projects as a scholar. During that time I mainly focused on the development, expression and purification of therapeutic fusion proteins as well as characterizing their effects on various cell lines.
During my time at the KCC I worked under the guidance of Dr. Darren Saunders using proteomics to investigate the role of a specific E3 ubiquitin-ligase.