Boston, Massachusetts, United States
- Characterized P. Pastoris fed-batch bioreactor fermentation process using particle size analyzer to couple enzyme activity with cell diameter-based induction timing - Doubled enzyme activity of expressed proteins by optimizing key fermentation parameters (feed profile, induction temperature, etc) using Design of Experiments (DoE) in JMP software - Minimized experimental inconsistencies and increased bioreactor homogeneity by modifying salts in classic, chemically defined medium - Extracted gDNA from yeast and performed library preparation to determine low copy number and verify mutations were not present - Prepared and ran several 2L fed-batch bioreactors in parallel using aseptic techniques across the process development workflow; produced over 100g dry E. Coli cell mass for a fellow intern project - Analyzed data and presented Summer project at company-wide seminar using Microsoft PowerPoint and Excel
- Optimized mixing and improved manufacturability in perfusion, microbial 1L bioreactor using JMP software - Validated efficacy of new reactor design through bio-analytical techniques such as SDS-PAGE and spectroscopy on perfusate samples - Prepared batch tickets, process media, and reactor tubing assembly for 1L bioreactor campaign and maintained a proper GMP environment during the campaign - Collaborated with associate engineers to design downstream unit operations (TFF, purification columns, etc) - Drafted Standard Operating Procedure (SOP) for U-Value testing; scripted data collection program in Python to process reactor data and calculate heat transfer and mixing capabilities - Communicated technical information and data to manufacturing partners and peers clearly and effectively using Google Workspace (Slides, Docs, Sheets, etc.)