Vienna, Vienna, Austria
Current occupation: Senior Sales Application Specialist for LC-MS at Waters Corporation Previous career: 2019-2025 Senior Field Service Engineer for LC-MS at Waters Corporation 2017-2019: Analytical Chemist/Postdoctoral Researcher at Karolinska Institute, Stockholm, Sweden, Department of Medical Biochemistry and Biophysics in the field of targeted lipidomics and metabolomics. My main focus in this role was the development, optimization and validation of comprehensive LC-MS/MS platforms (chiral as well as achiral) for profiling of lipid mediators and applying these platforms for biomarker identification. 2014-2017: PhD at the University of Copenhagen, Department of Pharmacy. Rearch Focus: Automation of a novel sample preparation method called electromembrane extraction to LC-MS. 2012-2014: Lab technician at Vela Labs, Vienna, Austria. Characterization services for biopharmaceuticals in a GMP compliant environment. During those one and a half years I was able to learn about regulatory and quality aspects in the various phases of the life-cycle of a pharmaceutical drug. My tasks included both, lab work (development and validations of HPLC methods and cell based assays for biosimilars) as well as the drafting and reviewing of various QC relevant documents such as GMP-compliant SOPs, method development- and validation plans and various reports for clients. 2007-2012: Bachelor and Master Degree in Medical and Pharmaceutical Biotechnology at IMC-FH Krems, Austria
Application Specialist for LC-MS (Liquid Chromatography- Mass Spectrometry)
Repair, maintenance and customer training on Waters LC-MS/MS an GC-MS/MS instruments
Development and validation of quantitative LC-MS/MS platforms for comprehensive lipid mediator profiling in biological samples Biomarker identification in asthma and COPD Gained expertise: Metabolomics/Lipidomics Bioanalysis targeted and non-targeted LC-MS chiral chromatography UHPLC automated solid-phase extractions Selected publications: 1. A LC-MS/MS workflow to characterize 16 regio- and stereoisomeric trihydroxyoctadecenoic acids / David Fuchs, Mats Hamberg, C. Magnus Sköld, Åsa M. Wheelock, Craig E. Wheelock, 2018 Published in the Journal of Lipid Research, DOI: 10.1194/jlr.D087429 2. Lipid Mediator Quantification in Isolated Human and Guinea Pig Airways: An Expanded Approach for Respiratory Research / Johan Kolmert, Alexander Fauland, David Fuchs, Jesper Säfholm, Crisina Gomez, Mikael Adner, Sven-Erik Dahlén, Craig E. Wheelock, 2018 Published in Analytical Chemistry, DOI: 10.1021/acs.analchem.8b01651
Investigation of the potential of a novel sample preparation method called electromembrane extraction for high throughput applications in combination with LC-MS Gained expertise: mass spectrometry HPLC automation of sample preparation techniques drug metabolism Selected publications: 1. Fully Automated Electro Membrane Extraction Autosampler for LC-MS Systems Allowing Soft Extractions for High-Throughput Applications. / Fuchs, David; Pedersen-Bjergaard, Stig; Jensen, Henrik; Rand, Kasper Dyrberg; Hansen, Steen Honoré; Petersen, Nickolaj J. In: Analytical Chemistry, Vol. 88 No. 13, 29.05.2016, p. 6797−6804. 2. Real Time Extraction Kinetics of Electro Membrane Extraction Verified by Comparing Drug Metabolism Profiles Obtained from a Flow-Flow Electro Membrane Extraction-Mass Spectrometry System with LC-MS. / Fuchs, David; Jensen, Henrik; Pedersen-Bjergaard, Stig; Gabel-Jensen, Charlotte; Hansen, Steen Honore; Petersen, Nickolaj Jacob. In: Analytical Chemistry, Vol. 87, No. 11, 02.06.2015, p. 5774-81.
Tasks: Planning and execution of QC relevant assay (Development, Qualification and Validation) as "first operator and "second operator" Development and validation of HPLC methods (reversed phase, size exclusion, ion exchange) Development of and validation of immunological assays including ELISA, FACS assays and cell based assays Realization of method validations as validation team leader Preparation of method development reports, instrument qualification plans, validation plans and reports as well as SOPs GMP compliant documentation
Master-Thesis: "Establishment of an expression platform-compatible silent chimeric human/mous IgG format" Tasks: Cloning of mammalian expression vectors Primer design and site directed mutagenesis Transient transfection of serum-free suspension adopted mammalian cells Optimization of antibody expression in mammalian cell culture (CHO and HKB 11 cells) Optimization of antibody purification strategies using protein A and protein G affinity chromatography Affinity determination of antibodies to different Fc receptors by surface plasmon resonance including method optimization Establishment of an ELISA method for the affinity determination of purified antibodies to proteins of the complement system