Daniel Ziemianowicz, PhD

3D Cell Culture Automation | Supporting NAMs Development | Regulatory Strategy | Biotech + Hardtech

Hamburg, Hamburg, Germany

About

Molecular biologist by training, currently working on the reproducibility problem in organoid automation. The core question: what does it actually take to make automated 3D culture data trustworthy enough for pharma decision-making? From my experience in electron microscopy, structural biology, mass spectrometry, and proteomics, I've learned to care about what a measurement is actually telling you, and where it breaks down. A relevant skill when you're trying to define acceptance criteria for a live biological system running on a robot. At MO:RE, I'm working on the process control around automated organoid workflows: QC criteria, imaging, protocol optimization and translating that into regulatory expectations for NAMs. The interesting part is designing the objective functions: what gets measured, what it means, and what "decision-grade data" actually requires from a 3D culture system. How can we build the infrastructure to make the biological model's output credible?

Experience

  • Systems Biology Scientist at mo:re

  • Doctoral Researcher | Structural Mass Spectrometry at University of Calgary

    Developed and applied novel biochemical and analytical methods for protein structure characterization via mass spectrometry. Supervised by Prof. Dr. David Schriemer. ▷ Innovated new experimental methods for protein structure analysis, resulting in a four-fold increase in data output compared to the previous design. Applied to the analysis of a pharmaceutical drug–­­protein and host-pathogen protein-protein interaction systems. Method was adopted as an approach for antibody epitope discovery in pharmaceutical industry (e.g. GSK, BMS) ▷ Co-developed algorithms for software for statistical evaluation of novel data, resulting in an approx. 10-fold increase in resolving amino-acid modifications ▷ Collaborated with chemists and biologists in industry (Thermo Fischer & Pfizer) and external research groups. Resulting in 5 first-author and 3 co-author publications ▷ Oversaw operation and maintenance of a flagship mass spectrometer instrument used by lab members

  • Instructor & Teaching Assistant | iGEM Team Calgary at University of Calgary

    Instructor and TA of Calgary’s international Genetically Engineered Machines (iGEM) team, an international research competition in applied synthetic biology with a focus on interdisciplinary collaboration. The project proposed a solution for the management of ionizing radiation experienced by astronauts. Supervised by Dr. Mayi Arcellana-Panlilio ▷ Recruited, led and mentored a multidisciplinary team of 15 undergraduate students in a synthetic biology competition ▷ Taught complex synthetic biology principles to an international team of students of varying backgrounds and skill sets ▷ Led an initiative to establish iGEM as a formal program at University of Calgary after a one year hiatus ▷ Project was nominated for Best Applied Design, and awarded Best Integrated Human Practices, demonstrating project excellence and practical considerations For more information and project details check out: http://2016.igem.org/Team:UofC_Calgary

  • Lifeguard, Swimming Instructor at The City of Calgary

    Responsibilites include instructing swimming lessons for both children and adults, monitoring patron safety and providing first-aid, customer service, maintaining a public swimming pool and recreational facility, and performing administrative duties. Certified as an Advanced Swimming Pool Operator.

  • Team Lead | iGEM Team Calgary at University of Calgary

    Team lead of Calgary’s Genetically Engineered Machines (iGEM) research team, an international research competition in synthetic biology with a focus on interdisciplinary collaboration. Our project addressed the misdiagnosis of infectious diseases. Supervised by Dr. Anthony Schryvers. ▷ Led a team of 13 peers from a diverse set of backgrounds in a competitive environment ▷ Managed a complex project with 4 sub-projects and performed strategic planning ▷ Fund-raised and recruited stakeholders providing CAD $10k+ in laboratory resources ▷ Co-organised and moderated an international, two-day workshop ▷ Engaged with legislative bodies on policy development ▷ Presented our project at the UN Biological Weapons Convention (Geneva, Switzerland) by invitation of the Canadian Delegation ▷ Participated in a joint Canada-US discussion on synthetic biology policy For more information and project details check out: http://2014.igem.org/Team:Calgary

  • Research Scientist (EIPOD) | Structural & Systems Biology at EMBL

    Studying the effects of Influenza A Virus infection on the human nuclear pore complex and viral replication. Supervised by Dr. Jan Kosinski. ▷ Employed cutting-edge technologies of cryo-electron microscopy, mass spectrometry proteomics, fluorescent light microscopy and computational modelling ▷ Optimised workflow process resulting in 3x increase in throughput ▷ Produced a previously unknown structure of the human nuclear pore complex during Influenza A Virus infection ▷ Collaborated with multidisciplinary computational and lab-based teams ▷ Statistically analysed large data sets e.g. 700+ 3D electron tomograms and proteomics data with 6000+ proteins with multiple dimensions over 5 time-points ▷ Utilised a high-performance compute cluster for data analysis and modelling ▷ Effectively communicated complex scientific concepts at three international conferences, reaching a cumulative audience 300+ experts ▷ Advised our Proteomics Core Facility in redesigning their cross-linking analysis service, resulting in 2x data output ▷ Recruited and mentored a Master's student through their research journey; mentored 2 PhD students in technical aspects and communication ▷ Acted as Postdoctoral Fellow Representative (Apr 2021 - Aug 2022) at the CSSB ▷ Initiated and led a 1.5–day professional development retreat, to counteract our reduced networking opportunities due to the pandemic for my 2019 EIPOD cohort