New York, New York, United States
As a molecular virologist with over a decade of dedication to the field, I possess comprehensive expertise in virology, viral-host interactions, and the genetic engineering of viruses. My work in developing recombinant viruses has significantly advanced the understanding of emerging respiratory viruses, elucidated virus-host interactions, and explored viral tropism. My proficiency in next-generation sequencing (NGS) includes extensive experience with various library preparation methods, targeted and sequencing runs using platforms such as 10x Genomics single cell, Illumina, and Nanopore. These skills have enabled detailed viral genome analysis and high-throughput sequencing of host responses, providing critical insights into RNA virus behavior. Specializing in RNA viruses, particularly in the engineering of enteroviruses and paramyxoviruses, I bring invaluable skills and insights to research teams dedicated to advancing the field of virology.
Dr. Benhur Lee's Lab, Icahn School of Medicine at Mount Sinai, New York, NY 1) Established BSL2-compatible surrogate virus neutralization assays during the COVID-19 pandemic - Generated surrogate CoV2-S pseudotyped particles based on vesicular stomatitis virus using reverse-genetics. - Developed a Sendai virus protein expression system and purified RBD-Fc protein Conducted serum neutralization assays on emergent viruses. 2) Antibody discovery in human blood from full-length single B cell transcriptomics - Enriched B cells and conducted fluorescence-activated cell sorting (FACS) for subjects vaccinated with MMR. - 10X Chromium library preparation for long-read single-cell transcriptome sequencing. 3) Investigated the nonstructural roles of the viral matrix protein from human parainfluenza virus type 3 - Creating a recombinant HPIV3 vector bearing different viral matrix mutants. - Utilizing CRISPR knock-in strategy to introduce regulatory elements into endogenous loci, generating cell lines for subsequent virology studies. - Assessing host and viral translational status using puromycin labeling, polysome profiling, and RNA-seq.
Dr. Benhur Lee's Lab, Icahn School of Medicine at Mount Sinai, New York, NY
Dr. Shin-Ru Shih's Lab, Chang Gung University, Taiwan - Employed genome-wide CRISPR screens to identify host protein acyl-coenzyme A synthetase long-chain family member 4 (ACSL4) plays a role in viral replication.